Sequences from confirmation amplicon


Confirmation sequences for line 571F07 and hit At3g06550 are shown.
Line ID 571F07
Locus At3g06550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2041078
Primers used for PCR MP35 - 8474, show primer details
   
Sequence Name 09-K023022-022-571-F07C-8409
GenBank Accession LN489996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K023022-022-571-F07C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAGCCATTGGTAATATTCTC
CACCTATGTATTGAGGAAATTTAGCCGCCACATCATCTGTAGACCAAGAATATGTTAGAA
TTTCAAGTAGGTGACAGCCAAAAATAAGAAAAAAGCCGGGAGGACATAGCTCTTACCTGT
GCAAACCTTGCAAGGCTAAAGTCCTTGCGAATGTAATAATAAGAAAAATTCCCAAATCCA
GTCATCCATACATAGCAAGCAATGAAAACACGGATCGCATTATAGTACTCTGCAGCAGCA
AAGTAGTGGTACATCAAGAAAAGGACCTACAACACCAGAATAAGAAAACAATGATGAGGT
AGCGGCAAATGAAAAGGCAGATAAAACACTAACATAAAAGTTAAATGACAAACCTGCATC
CAGCCTTTCCACTCCTCGGTTTGATGCCTATTCAAGTACATGATGGCTTTTCCGCTGAAT
GGTGATTTATCAGTATGTATCGTGAATGAAGTTATCGCTGAAACGATGATGAG
   
Sequence Name 10-K023022-022-571-F07C-MP35
GenBank Accession LN490469 [GenBank]
Sequencing primer gene specific primer, MP35: 5'-CCTTTACTTCCTTCTCATCATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K023022-022-571-F07C-MP35
CGATAACTTCATTCACGATACATACTGATAAATCACCATTCAGCGGAAAAGCCATCATGT
ACTTGAATAGGCATCAAACCGAGGAGTGGAAAGGCTGGATGCAGGTTTGTCATTTAACTT
TTATGTTAGTGTTTTATCTGCCTTTTCATTTGCCGCTACCTCATCATTGTTTTCTTATTC
TGGTGTTGTAGGTCCTTTTCTTGATGTACCACTACTTTGCTGCTGCAGAGTACTATAATG
CGATCCGTGTTTTCATTGCTTGCTATGTATGGATGACTGGATTTGGGAATTTTTCTTATT
ATTACATTCGCAAGGACTTTAGCCTTGCAAGGTTTGCACAGGTAAGAGCTATGTCCTCCC
GGCTTTTTTCTTATTTTTGGCTGTCACCTACTTGAAATTCTAACATATTCTTGGTCTACA
GATGATGTGGCGGCTAAATTTCCTCAATACATAGGTGGAGAATATTACCAATGGCTAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTA

Last Updated on 06.12.2018 16:11