Sequences from confirmation amplicon


Confirmation sequences for line 572B09 and hit At1g01830 are shown.
Line ID 572B09
Locus At1g01830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:299826
Primers used for PCR 29ny - 8474, show primer details
   
Sequence Name 27-K114669-0022-572-B09C-29ny
GenBank Accession LN498508 [GenBank]
Sequencing primer gene specific primer, 29ny: 5'-AGTTTACAGGAAGATGGAAGACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114669-0022-572-B09C-29ny
GAACAAGCTTCGCAACGAGCAGTTGCAATCTGTGGCTAAGACGCTTAGTGAAGTGATAGA
GCTTGCAGAGCAGTGTTCAACTGACAAGTATGAAGGGAAGCTGCGTATGCAGAGCGATCT
AGATTCTTTATCCGGAAAACTGGATTTTGAATCTAAGAGACTGTGGGGTTTTGATCAAGA
CCGGCGTTCTCGGTGAAGCTACGTTACCTCTTTACATTTCAAGTTCATCGGAAACACCCA
AAATTTCCAGTTTAAAAGAACTTCTAGCGAGGCTACAGATCGGTCACTTGGAATCAAAAC
ACAATGCTCTCGAAAGCCTCCTTGGTGCAATGCAAGAAGATGAAAAGATGGTTCTTATGC
CTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CANAAATGTAG
   
Sequence Name 04-K114668-0022-572-B09C-8409
GenBank Accession LN488020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114668-0022-572-B09C-8409
TCCTGCAAGGCATAAGAACCATCTTTTCATCTTCTTGCATTGCACCAAGGAGGCTTTCGA
GAGCATTGTGTTTTGATTCCAAGTGACCGATCTGTAGCCTCGCTAGAAGTTCTTTTAAAC
TGGAAATTTTGGGTGTTTCCGATGAACTTGAAATGTAAAGAGGTAACGTAGCTTCACCGA
GAACGCCGGTCTTGATCAAAACCCCACAGTCTCTTAGATTCAAATCCAGTTTTCCGGATA
AAGAATCTAGATCGCTCTGCATACGCAGCTTCCCTTCATACTTGTCAGTTGAACACTGCT
CTGCAAGCTCTATCACTTCACTAAGCGTCTTAGCCACAGATTGCAACTGCTCGTTGCAGA
GCTTGTTCTTGGAGAAGCAAGGGTGGCTTGAGAGGTCAGATAAACAAGCTGGTATCTGTT
CGATCTTCGAGATGATCGTCTTCCATCT

Last Updated on 06.12.2018 16:11