Sequences from confirmation amplicon


Confirmation sequences for line 572H01 and hit At1g02500 are shown.
Line ID 572H01
Locus At1g02500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:520141
Primers used for PCR IN06 - 8474, show primer details
   
Sequence Name 59-K033552-022-572-H01C-8409
GenBank Accession LN509912 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K033552-022-572-H01C-8409
TTACAATTGAATATATCCTGGAAATGGCTAATATTCAATATTCGTAAGCCGCAGTTTTCA
AGAACCTTCCGTTTCCTCCTCTCTTTAGGTCAAGGTTAATTGTCATCATTCCTGGTCTGA
AGTCGAAGCTCTCCTTCACAATCTTTAGTATCTCCTTGTCTGGAATCAATCCAGTCTCAT
AAGTGTCCACAAAGACAGACAATGGCTCAGGGACTCCAATGGCGTATGAGACCTGAACAA
GAGCCCTACGAGCCATCCCGTTAGCCACAACGCTCTTAGCTGCTTGTCTCACAATGTAGG
CTCCGCTTCTGTCGACCTTGGTTGGGTCTTTACCTGAGAATGCACCGCCTCCGTGAGCTC
CCCATCCACCGTAAGTGTCGATGATGATCTTACGCCCGGTGAGACCAGCGTCTCCATGGG
GTCCACCGATGACGAATCGGCCTGAAGGGTTCAAGTGGAAGATGGTTTTCCTCGTCTA
   
Sequence Name 59-K033552-022-572-H01C-IN06
GenBank Accession LN509913 [GenBank]
Sequencing primer gene specific primer, IN06: 5'-CTAGACGAGAAAACCATCTTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K033552-022-572-H01C-IN06
CCATGGAGACGCTGGTCTCACCGGGCGTAAGATCATCATCGACACTTACGGTGGATGGGG
AGCTCACGGAGGCGGTGCATTCTCAGGTAAAGACCCAACCAAGGTCGACAGAAGCGGAGC
CTACATTGTGAGACAAGCAGCTAAGAGCGTTGTGGCTAACGGGATGGCTCGTAGGGCTCT
TGTTCAGGTCTCATACGCCATTGGAGTCCCTGAGCCATTGTCTGTCTTTGTGGACACTTA
TGAGACTGGATTGATTCCAGACAAGGAGATACTAAAGATTGTGAAGGAGAGCTTCGACTT
CAGACCAGGAATGATGACAATTAACCTTGACCTAAAGAGAGGAGGAAACGGAAGGTTCTT
GAAAACTGCGGCTTACGAATATTGAATATTAGCCATTTCCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11