Sequences from confirmation amplicon


Confirmation sequences for line 573B03 and hit At1g15810 are shown.
Line ID 573B03
Locus At1g15810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5445227
Primers used for PCR 75zi - 8474, show primer details
   
Sequence Name 34-K126375-0022-573-B03C-75zi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 75zi: 5'-ATAAATTTGTGGACGTGTGAGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K126375-0022-573-B03C-75zi
ATTGTAGATGATGCCATATTGCCTATGTAGGATAAAGTTTTACGAAATTAGGATTAGTTA
ACTTATGTATTTATATTAAGTATGCATTGAACTCGTTTTCTTTTTCTTTTTTGGCTAGGA
TTGAAAATTATAATTTAATGTCCATTCATACAGTATTTACTGTCCATAATACTCCAAAAA
TTTGAAACAAATTATCCAATTATTAAATGGTCGCTAAATTCGAAAAACCTAAGGGCTTTG
TTGTGGGTACATGGGTCGAAAACAGAAAGATGAATTGGGCCTTTTCACATGTCCGTCATG
TTGCAATGTAAGAATTATATCCGGTTCTTAATCAGATTTTGCCTGACCATTTCTGATTCA
TAACTAACCGGAAAAGTCAACAGTTCAATGCACTTAAACCGGTTCTACCTTAATCTGTTA
TCGTCATCACTTACACTCTCTCGCAAACACCCAAAGCCCTAAAATCCTTAGGAGCAACCA
AAATCATGGCGCTTCATCTCGCCAGACGCCACCAAAGGTTACCATCAAACACATCTTTGA
TTCGATTTTTCTCCAATTCCTCATCGGACTCACCTCCCCAATTCACGAACGCACCGGTTG
AAAAACCGTCTCAGTCACCATCTTCTCCGTCACCGTCAGACTCTTCTCCGATCTTCGACA
TCAAGAACACTCTGAAACTGAAATTACAGAACCAGCAGGAGCAAGGAAGGAAACCATTTG
ACAGATCCGATGCACAATCTCAGAATCGCGGATCGACTAGTTTCTCCCAAAACTTGGGGA
AGAATCTCGCCAAGTTTCAGCGGAGATCCACCGTTCCACCTCCGAGGGATTCAGGCGGCG
TACAGCCTCCGTCTTATATTTCCTTAGCGGGCCTCTATAAGCAGAGCGCTGCAACAAACT
CATCGGATCCTAGAAATTCGAAAGCTCAGGGTTTCAATTCAAACACTGTAAG
   
Sequence Name 27-K126373-0022-573-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K126373-0022-573-B03C-8409
ATTGAGAACCATCCTTCTTTTTTCTCTCCTTCTGGCCTAAGCGTCCTTAGTTTCTCACCT
AATTCTTCTTCGTTATAAAACATTAAGAACTCCGTCATCGCCTCCTCTGTTTCACCTTCA
CTTCCCTTACTCTCATCCATTTGTGTTTCGAACAGGTATTTTGGCAATGCTTCACCTACA
GTGCTCTTGAAACTTCCTAAAACCCCTCCTCTCTTCCCCATGTTGTTTGAAGTGTTAATC
TGCGTCCTCAAATTCTTTAAAGTTTCACTTACAGTGTTTGAATTGAAACCCTGAGCTTTC
GAATTTCTAGGATCCGATGAGTTTGTTGCAGCGCTCTGCTTATAGAGGCCCGCTAAGGAA
ATATAAGACGGAGGCTGTACGCCGCCTGAATCCCTCGGAGGTGGAACGGTGGATCTCCGC
TGAAACTTGGCGAGATTCTTCCCCAAGTTTTGGGAGAAACTAGTCGATCCGCGATTCTGA
GATTGTGCATCGGATCTGTCAAATGGTTTCCTTCCTTGCTCCTGCTGGTTCTGTAATTTC
AGTTTCAGAGTGTTCTTGATGTCGAAGATCGGAGAAGAGTCTGACGGTGACGGAGAAGAT
GGTGACTGAGACGGTTTTTCAACCGGTGCGTTCGTGAATTGGGGAGGTGAGTCCGATGAG
GAATTGGAGAAAAATCGAATCAAAGATGTGTTTGATGGTAACCTTTGGTGGCGTCTGGCG
AGATGAAGCGCCATGATTTTGGTTGCTCCTAAGGATTTTAGGGCTTTGGGTGTTTGCGAG
AGAGTGTAAGTGATGACGATAACAGATTAAGGTAGAACCGGTTTAAGTGCATTGAACTGT
TGACTTTTCCGGTTAGTTATGAATCAGAAATGGTCAGGCAAAATCTGATTAAGAACCGGA
TATAATTCTTACATTGCAACATGACGGACATGTGAAAAAGCCCAAATCATCTTTCTGTTT
T

Last Updated on 06.12.2018 16:11