Sequences from confirmation amplicon


Confirmation sequences for line 573C05 and hit At5g57123 are shown.
Line ID 573C05
Locus At5g57123 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23127226
Primers used for PCR BM16 - 8474, show primer details
   
Sequence Name 18-K116018-0022-573-C05C-BM16
GenBank Accession LN494451 [GenBank]
Sequencing primer gene specific primer, BM16: 5'-ATGCACCAACCAAATATCCTATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116018-0022-573-C05C-BM16
TCAAATTTGCTCTTTCATATAAGCCTCCGCAAGAAGTTTCTCAGAAACAGTTTTCTCAAG
AAAATAATTGAATTTTCCTCTAAAGCTCACAAAATTCTCTGATCTTCCAAACAAAATAAA
CCCATTACAGAATTTTTACAAAACTAAAACTTCCCTAGCTACCAAAAGAAAAAGAGAGGC
AAGACTCAGATCTGAGAGAGAAAAACAATGTGTTTGGTGTGTTTATGTGATGAGGAAGAG
ACAGAGTTAGGGAGACAACAAGCACCTGGATCGTGTCCTTATTGTGGAGGTAAAGTGCAA
ATGCTTGATGTCGAAAGAAAATGGATGTTCTGTTTCGTTCCTCTTTGTTTCAAGATCAAG
AGGAAGTACTCGTGTTCTTCTTGTGATCGTCGTCTCGTTTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K116017-0022-573-C05C-8409
GenBank Accession LN488493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116017-0022-573-C05C-8409
ATTTACATTGAATAATCCTGAAACGAGACGACGATCACAAGAAGAACACTATAACTTCCT
CTTGATCTTGAAACAAAGAGGAACGAAACAGAACATCCATTTTCTTTCGACATCAAGCAT
TTGCACTTTACCTCCACAATAAGGACACGATCCAGGTGCTTGTTGTCTCCCTAACTCTGT
CTCTTCCTCATCACATAAACACACCAAACACATTGTTTTTCTCTCTCAGATCTGAGTCTT
GCCTCTCTTTTTCTTTTGGTAGCTAGGGAAGTTTTAGTTTTGTAAAAATTCTGTAATGGG
TTTATTTTGTTTGGAAGATCAGAGAATTTTGTGAGCTTTAGAGGAAAATTCAATTATTTT
CTTGAGAAAACTGTTTCTGAGAAACTTCTTGCGGAGGCTTATATTGAAAGAGCAAATTTT
GAAGTCTTATTTTTTTAAGTCGCTAAGATAGGATATTTGGTTTGGTGCATA

Last Updated on 06.12.2018 16:11