Sequences from confirmation amplicon


Confirmation sequences for line 573F09 and hit At5g62160 are shown.
Line ID 573F09
Locus At5g62160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24960880
Primers used for PCR 57iv - 8474, show primer details
   
Sequence Name 37-K112810-0022-573-F09C-57iv
GenBank Accession LN502674 [GenBank]
Sequencing primer gene specific primer, 57iv: 5'-AATGTTGATTGAATCCTTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112810-0022-573-F09C-57iv
GTAGACTCTGCCGGTGAGTACAGGCGGAGAGGAGGAACATGCTCACACTGGCTCTGCTCA
TACTCATGCCTCACAAGGACATTCTCATGGCTCTCTTCTTATTCCCCAAGATGATGATCA
CATTGATATGAGGAAAAAGATTGTAACTCAGGTACGTTAAAGTTTTTGGAAATTCTTGAT
TTAGATAAAGACAAACCCGTATTATTAGTCACATGTTGTCTATTTTTCTTATGGTGTTAG
ATATTGGAATTGGGGATTGTGGTTCATTCAGTGATAATAGGAATATCTCTAGGAGCATCT
CCAAGTGTGAGCACAATAAAGCCTCTCATAGCTGCAATTACTTTGAGGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGATTATTA
   
Sequence Name 12-K112809-0022-573-F09C-8409
GenBank Accession LN491653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112809-0022-573-F09C-8409
TGCTCACACTTGGAGATGCTCCTAGAGATATTCCTATTATCACTGAATGAACCACAATCC
CCAATTCCAATATCTAACACCATAAGAAAAATAGACAACATGTGACTAATAATACGGGTT
TGTCTTTATCTAAATCAAGAATTTCCAAAAACTTTAACGTACCTGAGTTACAATCTTTTT
CCTCATATCAATGTGATCATCATCTTGGGGAATAAGAAGAGAGCCATGAGAATGTCCTTG
TGAGGCATGAGTATGAGCAGAGCCAGTGTGAGCATGTTCCTCCTCTCCGCCTGTACTCAC
CGGCAGAGTCTTACCCTCTTTCGCCAAGCGAGATCTGTTCAAATACCCTGAAGCAAAGGA
TTCAATCAACAT

Last Updated on 06.12.2018 16:11