Sequences from confirmation amplicon


Confirmation sequences for line 573G03 and hit At2g43570 are shown.
Line ID 573G03
Locus At2g43570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18077002
Primers used for PCR 38iv - 8474, show primer details
   
Sequence Name 82-K113077-0022-573-G03C-38iv
GenBank Accession LN514212 [GenBank]
Sequencing primer gene specific primer, 38iv: 5'-TCACGGATCCATCATCATCTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K113077-0022-573-G03C-38iv
AGACACAAAAACAATGGCTAAACCCACATCACGAAATGACCGATTTGCCCTTTTCTTCAT
AACCCTAATTTTCCTGATCCTAACCGTTTCCAAACCGGTCGCCTCTCAAAACTGTGGCTG
CGCCTCTGACTTCTGTTGCAGCAAATATGGCTACTGTGGTACGACCGATGAGTTCTGCGG
CGAGGGCTGCCAGGCAGGACCTTGCCGGAGCAGTGGAGGCGGTGGAGATCCTGCTGTTTC
ACTAGAAGGAACGGTGACACCTGACTTCTTTAACTCTATACTAAACCAAAGAGGTGATTG
TCCTGGCAAAGGATTCTACACTCACGACACCTTCATGGCCGCAGCTAATTCTTACCCTAG
CTTCGGTGCTTCCATCTCCAAACGCGAAATCGCGGCGTTTTTCGCTCACGTAGCCCAAGA
AACAGGGTGTAAGTAAATTTTCTCATAACATAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 55-K113075-0022-573-G03C-8409
GenBank Accession LN508865 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K113075-0022-573-G03C-8409
GCATTTTGTATGAGAAATTTACTTACACCCTGTTTCTTGGGCTACGTGAGCGAAAAACGC
CGCGATTTCGCGTTTGGAGATGGAAGCACCGAAGCTAGGGTAAGAATTAGCTGCGGCCAT
GAAGGTGTCGTGAGTGTAGAATCCTTTGCCAGGACAATCACCTCTTTGGTTTAGTATAGA
GTTAAAGAAGTCAGGTGTCACCGTTCCTTCTAGTGAAACAGCAGGATCTCCACCGCCTCC
ACTGCTCCGGCAAGGTCCTGCCTGGCAGCCCTCGCCGCAGAACTCATCGGTCGTACCACA
GTAGCCATATTTGCTGCAACAGAAGTCAGAGGCGCAGCCACAGTTTTGAGAGGCGACCGG
TTTGGAAACGGTTAGGATCAGGAAAATTAGGGTTATGAAGAAAAGGGCAAATCGGTCATT
TCGTGATGTGGGTTTAGCCATTGTTTTTGTGTCTGTGATGCAGACTGCAGAGAGAGATGA
TGAACATTAGCTTCGTGTTTTATAGATGATGAGGNAATCCGTGA

Last Updated on 06.12.2018 16:11