Sequences from confirmation amplicon


Confirmation sequences for line 574C03 and hit At1g74130 are shown.
Line ID 574C03
Locus At1g74130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27875484
Primers used for PCR KG33 - 8474, show primer details
   
Sequence Name 84-K038033-022-574-C03C-KG33
GenBank Accession LN514390 [GenBank]
Sequencing primer gene specific primer, KG33: 5'-ACGTATACACACGCTGATCACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K038033-022-574-C03C-KG33
TCATATCATCTTGAATATGATGGGGCTTTGCTACTTCGGAGCCAGAGTAAGATTTTTTCC
TTGTTTCCATATATTAATTTTCGTAAACGAAATGAGAAATGCTTTCATTTGTTTCCTATG
AAGTATGAACCGGCTACTTACACTTAATCTGGCTCATCTAAGGACCGTCCTTAGTTCGTT
TAAAAAGAAAAACTTGCTACCTATTGATCTTTATATCGAATCCCATTTGTTCTTAGATTG
CAAGAAGCTTTGGGCCGCGCTACCTTTTGAAGCTCTACTTTGCTGGAGCACTTGGTGGCT
CTGTTTTCTTCTTGAGTAGTCATGCTCTCTCGGTTATATCACTCAAGGTATAGTGCAACA
AGCATAATTTTCTCATTTTCCTAACCATGCTAGGTCCACAGATGTGTAGTTTTTCCAGTG
CATTTGTACTACGTACATGTTCTCTATGTGTTTGTGAGGTTTAGACGAAATGCTTTACCT
TTGTTATTGGTTAATCAATCATACAGGGCCAAAGAGTTGTCCCTAAGGATCAATTAAAAG
TCCCCATTGGGAAGCTGGTATGTTTTTTCACTCATCCAGTTAAATTATTTTTTAAAAGTA
TAAACAGACTGGAACAGAGATGCACACGCTCATGTTTTGGATGGTCAAACTCTTTCCAAT
TTGTGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
AATCTTATTTCGACGTGTC
   
Sequence Name 28-K038033-022-574-C03C-8409
GenBank Accession LN498754 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K038033-022-574-C03C-8409
GCCATTTAAATTGAATATATCCTGTACACAAATTGGAAAGAGTTTGACCATCCAAAACAT
GAGCGTGTGCATCTCTGTTCCAGTCTGTTTATACTTTTAAAAAATAATTTAACTGGATGA
GTGAAAAAACATACCAGCTTCCCAATGGGGACTTTTAATTGATCCTTAGGGACAACTCTT
TGGCCCTGTATGATTGATTAACCAATAACAAAGGTAAAGCATTTCGTCTAAACCTCACAA
ACACATAGAGAACATGTACGTAGTACAAATGCACTGGAAAAACTACACATCTGTGGACCT
AGCATGGTTAGGAAAATGAGAAAATTATGCTTGTTGCACTATACCTTGAGTGATATAACC
GAGAGAGCATGACTACTCAAGAAGAAAACAGAGCCACCAAGTGCTCCAGCAAAGTAGAGC
TTCAAAAGGTAGCGCGGCCCAAAGCTTCTTGCAATCTAAGAACAAATGGGATTCGATATA
AAGATCAATAGGTAGCAAGTTTTTCTTTTTAAACGAACTAAGGACGGTCCTTAGATGAGC
CAGATTAAGTGTAAGTAGCCGGTTCATACTTCATAGGAAACAAATGAAAGCATTTCTCAT
TTCGTTTACGAAAATTAATATATGGAAACAAGGAAAAAATCTTACTCTGGCTCCGAAGTA
GCAAAGCCCCATCATATTCAAGATGATATGAGTGGCTCCAACATGACTGAAACCCGAAGT
GATCA

Last Updated on 06.12.2018 16:11