Sequences from confirmation amplicon


Confirmation sequences for line 574F03 and hit At1g74170 are shown.
Line ID 574F03
Locus At1g74170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27893269
Primers used for PCR 15gw - 8474, show primer details
   
Sequence Name 39-K112877-0022-574-F03C-8409
GenBank Accession LN503412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112877-0022-574-F03C-8409
CATTTCAATTGATATTTTCCTGAACGTGTCAGTCAACAAATTCAATCATCTCTTTCTTCA
GAACTTTGGGTGGATACTCCCTCATTTAGTCTGTGTGAATTTAGCCTATAATGGTTTTCA
AGGAAATCTGCCATCTTCTCTGGATAACATGAAGAGTATTGAATTTCTAGATTTGTCTCA
CAACAGGTTCCACGGGAAGCTACCAAGAAGATTTTTAAAGGGTTGTTATAACTTAACAAT
CTTGAAGCTGTCACACAACAAACTAAGTGGAGAGGTTTTTCCAGAAGCAGCCAACTTTAC
TCGTTTATGGGTGATGTCTATGGATAACAATCTGTTTACAGGAAATATTGGAAAAGGTTT
TCGGAGCCTACCATCTTTGAATGTACTTGACATTTCAAACAACAAACTCACAGGTGTTAT
TCCAAGTTGGATTGGCGAACGTCAGGGATTATTCGCACTGCAGCTTTCAAACAACATGTT
GGAAGGTGAAATACCTACTTCCTTGTTCAACATATCCTATCTTCAGCTACTGGACCTCTC
TTCAAACAGGTTATCTGGG
   
Sequence Name 76-K112878-0022-574-F03C-15gw
GenBank Accession LN513426 [GenBank]
Sequencing primer gene specific primer, 15gw: 5'-CCCAGATAACCTGTTTGAAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K112878-0022-574-F03C-15gw
GTATTTCACCTTCCACATGTTGTTTGAAAGCTGCAGTGCGAATAATCCCTGACGTTCGCC
AATCCAACTTGGAATAACACCTGTGAGTTTGTTGTTTGAAATGTCAAGTACATTCAAAGA
TGGTAGGCTCCGAAAACCTTTTCCAATATTTCCTGTAAACAGATTGTTATCCATAGACAT
CACCCATAAACGAGTAAAGTTGGCTGCTTCTGGAAAAACCTCTCCACTTAGTTTGTTGTG
TGACAGCTTCAAGATTGTTAAGTTATAACAACCCTTTAAAAATCTTCTTGGTAGCTTCCC
GTGGAACCTGTTGTGAGACAAATCTAGAAATTCAATACTCTTCATGTTATCCAGAGAAGA
TGGCAGATTTCCTTGAAAACCATTATAGGCTAAATTCACACAGACTAAATGAGGGAGTAT
CCACCCAAAGTTCTGAAGAAAGAGATGATTGAATTTGTTGACTGACACGTTCAGGAAAAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGGTTATT

Last Updated on 06.12.2018 16:11