Sequences from confirmation amplicon


Confirmation sequences for line 574F05 and hit At5g19990 are shown.
Line ID 574F05
Locus At5g19990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6753978
Primers used for PCR CH38 - 8474, show primer details
   
Sequence Name 37-K106590w-0022-574-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K106590w-0022-574-F05C-8409
GGAATGAGCCTTTACATTGAATAATCCTGGATATAGACTTCATGTAGATTTCCCCTTGAG
ATTTCAACGAATCATCTCACCTATTTGTAATTTGCTCCTTACATGAGACTTGGCACTGTG
GCCTAAATGCTAAGCACTATGACAAAGAATACTTTATAGAACGATATTCATTCACAACGG
AACCATATTCATTGTAGCAACAAAGAACACTTTCCCCATTTAACTTAACAGCTCATGTCC
ATTTTATAGCAACTAATGGCGGACATGTTAAAAGATGTTATCTTATCAACAGTTCCATAT
TTTCCTATCAACCACTATTACCATGCTTGAAATTTAAAATAGGGACTACCATATACTAAC
CTTGCCATCACAAAAAGTTCTCTGACCATTCTAGAACCTTCTCCAATGTATTTCTGGACA
AGCTCGGAACCAGAAACTCTTATGAAAGTACAGTCAGTGTGATGTGCAACAGCCCGAGCC
AATAGTGTCTTCCCAGTTCCAGGTGGACCGTATAACAACACACCCTAGAAAGACAGAGAG
ATGAGATTACGTCTAATCCACTTAGCATCATCACAATATGGAGAAGAAGGTTTAAGATTC
AAGTGTACCTTTGGCTGCGCAATTCCAAGAGACTCAAACAATTCAGGATGCTTGATTGGC
AGCTCAATGACCTGATAAACCAGAAAAGATATAGATATTATATAAACATTAAAAGGATGA
AACAACAGAGGAGA
   
Sequence Name 40-K106591w-0022-574-F05C-CH38
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CH38: 5'-TCTCCTCTGTTGTTTCATCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K106591w-0022-574-F05C-CH38
TGAGCTGCCATCAAGCATCCTGAATTGTTCGATTCTCTTGGAATTGCGCAGCCAAAGGTA
CACTTGAATCTTAAACCTTCTTCTCCATATTGTGATGATGCTAAGTGGATTAGACGTAAT
CTCATCTCTCTGTCTTTCTAGGGTGTGTTGTTATACGGTCCACCTGGAACTGGGAAGACA
CTATTGGCTCGGGCTGTTGCACATCACACTGACTGTACTTTCATAAGAGTTTCTGGTTCC
GAGCTTGTCCAGAAATACATTGGAGAAGGTTCTAGAATGGTCAGAGAACTTTTTGTGATG
GCAAGGTTAGTATATGGTAGTCCCTATTTTAAATTTCAAGCATGGTAATAGTGGTTGATA
GGAAAATATGGAACTGTTGATAAGATAACATCTTTTAACATGTCCGCCATTAGTTGCTAT
AAAATGGACATGAGCTGTTAAGTTAAATGGGGAAAGTGTTCTTTGTTGCTACAATGAATA
TGGTTCCGTTGTGAATGAATATCGTTCTATAAAGTATTCTTTGTCATAGTGCTTAGCATT
TAGGCCACAGTGCCAAGTCTCATGTAAGGAGCAAATTACAAATAGGTGAGATGATTCGTT
GAAATCTCATGTATGAAATCTACATGAAGTCTATATCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA

Last Updated on 06.12.2018 16:11