Sequences from confirmation amplicon


Confirmation sequences for line 576A05 and hit At2g21550 are shown.
Line ID 576A05
Locus At2g21550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9229818
Primers used for PCR 27fp - 8474, show primer details
   
Sequence Name 64-K111394-0022-576-A05C-27fp
GenBank Accession LN511227 [GenBank]
Sequencing primer gene specific primer, 27fp: 5'-GTTGTCCATCTTCTTTTTCTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K111394-0022-576-A05C-27fp
AGACACACAAGTTATATTGTTTCCTGATTTGAGAGAGAGCAAAATATATTTAGTACTTAC
CGGGAAGGGTTTAGGGGAGATTTGAAGCTGTTTTTGTATAGCCTTGACGTGAGCTTTGTT
AATATAGGCTTGTCCAATCACATGGATGAAATCACCAGCTCCAAGATCTGTCAAGACAAG
AAACCATACAAAGGTTTCAACTCTTTTACTTTAGACTCAAGAGCTAATAAAATGTGTACA
TACCGCAAACATGAGCGATGATGCATGTCAAAAAAAGGACGTATACTGTTATCCAATGTC
CTATATCCAATGTCAAAAAAGGACGTATAGGAGGAGGACATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGNTTATTA
   
Sequence Name 48-K111393-0022-576-A05C-8409
GenBank Accession LN506534 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K111393-0022-576-A05C-8409
CTCCTCCTATACGTCCTTTTTTGACATTGGATATAGGACATTGGATAACAGTATACGTCC
TTTTTTTGACATGCATCATCGCTCATGTTTGCGGTATGTACACATTTTATTAGCTCTTGA
GTCTAAAGTAAAAGAGTTGAAACCTTTGTATGGTTTCTTGTCTTGACAGATCTTGGAGCT
GGTGATTTCATCCATGTGATTGGACAAGCCTATATTAACAAAGCTCACGTCAAGGCTATA
CAAAAACAGCTTCAAATCTCCCCTAAACCCTTCCCGGTAAGTACTAAATATATTTTGCTC
TCTCTCAAATCAGGAAACAATATAACTTGTGTGTCTCTAATGTATTAACGGTAAAAACAG
ATTTTGAAAATCAACCCTGAGAAAAAGAAAATGGACAA

Last Updated on 06.12.2018 16:11