Sequences from confirmation amplicon


Confirmation sequences for line 578A11 and hit At3g61700 are shown.
Line ID 578A11
Locus At3g61700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22837110
Primers used for PCR NG30 - 8474, show primer details
   
Sequence Name 40-K102366-0022-578-A11C-8409
GenBank Accession LN503662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K102366-0022-578-A11C-8409
TTACAATTGAATATGAGTATGAGTATGTTACACATATACGAGGGAGGACACAACATATTT
ACACTTAGATAGGGAGAGAGCGATGAAACCTACTTTTTAATAGTTAAAAATGACAAGAAG
GAGCTCAAATTTGTTTCTTACAAGTTCAATATCTTGTCGTGAAACTTTCCTACTGTCATT
GCTTGAAGCAGATACAGAACCAGAATCTTGTATAGGAGCATCTATTGATAGGTTGGTTTT
CTGGTCACCTTGAGAGTCATGTGAAGCTTGAGTGGAAGATTGTAGCTCCTGTGCGCACTG
CCATCAAATAATAGTTATTTTCTAAAGTTTCACTTTTTTGTAATCAAAATCTACAAGTAC
TGACACAGCTTAATTATTGCTAGTAAGGAAATTTAAACATTGTCCAACTTCTTATGTGAA
AGTGTTAACCTAATCCACCAATCTTTATAGCTATAAGGGACCAGAAACTACTTGTTAGCT
TACGATCAACATTGTCCAACTTCTTATGTGAAAGTGTTAACCTAATCCACCAATCTTTAT
AGCTACAAGGGACCAGAAACTAGTTGTTATCTTGCAAAAAGGAAAGGCATATCAGAAGTT
ACACAACTTTTGAAGAAGACTAAAGCAAATACAACAAAGGTAGAATAATTGTGTTATAGA
AGAACCTGTCCGGTCTAATTTGATCCAAAAAAAAAAAAAGAAAAACCTGTCCGTTCTTCA
TTACTGCTGAATGTAAAGTGAAAACATCCTTCATTC
   
Sequence Name 40-K102367-0022-578-A11C-NG30
GenBank Accession LN503663 [GenBank]
Sequencing primer gene specific primer, NG30: 5'-TGGTGCTAAAGAGAACACTTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K102367-0022-578-A11C-NG30
CAGTAATGAAGACGGACAGGTTCTTCTCTTTTTTTTTTTGGATCAAATTAAACCGGACAG
GTTCTTCTATAACACAATTATTCTACCTTTGTTGTATTTGCTTTAGTCTTCTTCAAAAGT
TGTGTAACTTCTGATATGCCTTTCCTTTTTGCAAAATAACAACTAGTTTCTGGTCCCTTG
TAGCTATAAAGATTGGTGGATTAGGTTAACACTTTCACATAAAAAGTTGGACAATGTTGA
TCGTAAGCTAACAAGTAGTTTCGGGTCCCTTATAGCTATAAAGATGGGTGGATTAGGTTA
ACACTTTCACATAAAAAGTTGGACAATGTTTAAATTTCCTTACTAGCAATAATTAAGCTG
TGTCAGTACTTGTAAATTTTGATTACAAAAAAGTGAAACTTTAGAAAATAACTATTATTT
GATGGCAGTGCGCACAGGAGCTACAATCTTCCACTCAAGCTTCACATGACTCTCAGGGTG
ACCAGAAAACCAACCTATCAATAGATGCTCCTATACAAAATTCTGGTTCTGTATCTGCTT
CAAGCAATGACAGTAGGAAAGTTTCACGACAAGATATTGAACTTGTAAAAAACAAATTTG
AGCTCCTTCTTGTCATTTTTAACTATAAAAAAGTAGGTTTCATCGCTCTCTCCCTATCTA
AGTGTAAATATGTTGTGTCCTCCCTCGTATATGTGTAACATACTCATACTCATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACGTGGATCAGCAATA

Last Updated on 06.12.2018 16:11