Sequences from confirmation amplicon


Confirmation sequences for line 578C12 and hit At4g02580 are shown.
Line ID 578C12
Locus At4g02580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1136521
Primers used for PCR 73qa - 8474, show primer details
   
Sequence Name 92-K115459-0022-578-C12C-73qa
GenBank Accession LN515207 [GenBank]
Sequencing primer gene specific primer, 73qa: 5'-GCTATAGCCTTTGCGTTATTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K115459-0022-578-C12C-73qa
ACTAGCTTTGCTGCATCAGATCCGGAATTGTGGTGGCTTAGGCTCACCAAGCAGTGTCTT
ATTCCCTCCTTCTGGTCCACACTTGATCCTCTTCGGGTTCTGAGTTCCATGCTGAAAAAC
AAAGATTAACATTATAAGAAGTTGGATTTGGATTTTGGATTTGGATTTTGGCTTAGAGGC
TACGTTCTCCAATTTGGAAAGTTAACCGAAACCAGATTGTTTTCCCCCACCGATGTAATG
CAATAGTAAGAGAAGAACATCAGGAAAGTATCCAAATGCAATGGGTTGTTAATGTCTTAC
CGGTGGCTTTTCTCCTTTTCTCAGCTTTTCAACAATTTCTACGACTTTCTCAGGTGTAAC
ATCTTCCTGTGTATTGTAGCATAAGACAGGATCCCCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGNTTATT
   
Sequence Name 43-K115458-0022-578-C12C-8409
GenBank Accession LN504947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115458-0022-578-C12C-8409
TTGGGGATCCTGTCTTATGCTACAATACACAGGAAGATGTTACACCTGAGAAAGTCGTAG
AAATTGTTGAAAAGCTGAGAAAAGGAGAAAAGCCACCGGTAAGACATTAACAACCCATTG
CATTTGGATACTTTCCTGATGTTCTTCTCTTACTATTGCATTACATCGGTGGGGGAAAAC
AATCTGGTTTCGGTTAACTTTCCAAATTGGAGAACGTAGCCTCTAAGCCAAAATCCAAAT
CCAAAATCCAAATCCAACTTCTTATAATGTTAATCTTTGTTTTTCAGCATGGAACTCAGA
ACCCGAAGAGGATCAAGTGTGGACCAGAAGGAGGGAATAAGACACTGCTTGGTGAGCCTA
AGCCACCACAATTCCGTGATCTTGATGCATGCTAAAGCTAAGTTCTGTTTGCCGCAGTTT
TATGTGGTTCAATAACGCAAGGNCTATAGCA

Last Updated on 06.12.2018 16:11