Sequences from confirmation amplicon


Confirmation sequences for line 579A02 and hit At5g37650 are shown.
Line ID 579A02
Locus At5g37650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14954178
Primers used for PCR 45xp - 8474, show primer details
   
Sequence Name 36-K119444-0022-579-A02C-45xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45xp: 5'-AAGCTATGGTTTTTCGGGTAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119444-0022-579-A02C-45xp
AGTTGATTCTTTCTGGATGTTGTAATCTGCAAGAGTGCGACCATCGTCAAGCTGTTTACC
AGCAAAGATTAGTCTCTGTTGATCCGGAGGGACTCCTTCCTTGTCTTGGATCTTAGCTTT
AACATTGTCAATAGTGTCTGAACTCTCAACTTCAAGGGTAATGGTTTTACCGGTAAGCGT
CTTGACAAATATCTGCATACCACCTCTCAACCTGAGAGCTAAGTGGAGTATTGAATCTTT
TTGGATACTGTAATCGGCCAAAGTACGGCCATCATCTAGCAGTTTACCGGAAAAGATGAG
TCTTTGCTGGTCTAGAGGAATCCCTTCTATGTCCTGGATCTTTGCTTTGACATTATTGAT
GGTGTCGGAGCTTACAACGTCAATGGTTATGGTCTTTTCTGTGAGAGTCTTTGCGTGAAT
TTGCATCGACATCTTTGAAACGAAAAAAAAAGTTTCAAGTAACAAAAGTTGAGAGAGGAA
GAATTCAGGAAACTTTTGCAGCAAGATTAGGGTTTACGAGAGATGAAGAATTGAATCGTG
AATAACAACAACTATGGTGAAAGATCTTCTTATATAGAGACTAGAGAGGCTTAAAGACTT
TGATTATTAAAAGTAAAAAGGAAAATTCCTAAAATGGAAATTGCTAAACCAATTCCTAAA
AAGGAAAATTGATTCCGCAGAAGTCAATTTACCTAAGGCTTAGGTTGGTTAAGCCAATCA
AATTCACTCTTTGGGAGCACTTTAAGATTTTCATCCACTGACCTCTCAATATCAACATTG
ATTCTCCACAACACATACTTACTTTCCCTTTTCATGCCATTGATCGCGTAGAGCCTCCAA
AGCAAACCCTTAAGAATCTACATGTAAATGCTTCATGTCCGGGAAATCTA
   
Sequence Name 04-K119443-0022-579-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119443-0022-579-A02C-8409
CATTTCATGTAGATTCCTTAAGGGTTTGCTTTGGAGGCTCTACGCGATCAATGGCATGAA
AAGGGAAAGTAAGTATGTGTTGTGGAGAATCAATGTTGATATTGAGAGGTCAGTGGATGA
AAATCTTAAAGTGCTCCCAAAGAGTGAATTTGATTGGCTTAACCAACCTAAGCCTTAGGT
AAATTGACTTCTGCGGAATCAATTTTCCTTTTTAGGAATTGGTTTAGCAATTTCCATTTT
AGGAATTTTCCTTTTTACTTTTAATAATCAAAGTCTTTAAGCCTCTCTAGTCTCTATATA
AGAAGATCTTTCACCATAGTTGTTGTTATTCACGATTCAATTCTTCATCTCTCGTAAACC
CTAATCTTGCTGCAAAAGTTTCCTGAATTCTTCCTCTCTCAACTTTTGTTACTTGAAACT
TTTTTTTTCGTTTCAAAGATGTCGATGCAAATTCACGCAAAGACTCTCACAGAAAAGACC
ATAACCATTGACGTTGTAAGCTCCGACACCATCAATAATGTCAAAGCAAAGATCCAGGAC
ATAGAAGGGATTCCTCTAGACCAGCAAAGACTCATCTTTTCCGGTAAACTGCTAGATGAT
GGCCGTACTTTGGCCGATTACAGTATCCAAAAAGATTCAATACTCCACTTAGCTCTCAGG
TTGAGAGGTGGTATGCAGATATTTGTCAAGACGCTTACCGGTAAAACCATTACCCTTGAA
GTTGAGAGTTCAGACACTATTGACAATGTTAAAGCTAAGATCCAAGACAAGGAAGGAGTC
CCTCCGGATCAACAGAGACTAATCTTTGCTGGTAAACAGCTTGACGATGGTCGCACTCTT
GCAGATTACAACATCCAGAAAGAATCAACTCTCCACTTGGTTCTCAGGTGGAGAGGTGGT
ATGCAGATTTTTGTCAGG

Last Updated on 06.12.2018 16:11