Sequences from confirmation amplicon


Confirmation sequences for line 581G09 and hit At5g58490 are shown.
Line ID 581G09
Locus At5g58490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23644316
Primers used for PCR 70wf - 8474, show primer details
   
Sequence Name 34-K118301-0022-581-G09C-70wf
GenBank Accession LN501590 [GenBank]
Sequencing primer gene specific primer, 70wf: 5'-AAAGGACTAGTGACCAACCTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118301-0022-581-G09C-70wf
TCCATAGACTGATTATAATCGCGGACATATTATTACCAAACAAAAGAAAAATGGTACATT
GAAAAAAACGATTTAATTAGGAACGTCAACAGCAGGATTCGAACCTGCGCAGGCAAAGCC
CAACAGATTTCGAGTCTGTCTCCTTAACCACTCGGACATATTGACATTTCGTGATAATTG
CAACACTGGATTTATATAAACAAAACTAAATCACTTAAAACACTTAAAGATATTCTCATG
TTCAAACTTTAAAGAAAAATCTCAGAGAGGCCAGATTAATAAATATTAATTGGATGTTCA
TACCATACCATAGGCCAGCTTATTTATTACAAAAGTGCAAAATCGTATATCATATGAAAT
GTTTCTGCTGCCTAGAGATTGTGTTAATTGCTCAATCAAACAGAGTTACAGAGGTAGGTA
TTAAGAGATAAATCCTTTGCTTTTAAGACTCTCCACACCTTCCTTGATGATTTCCTCCAT
GGAAATGAACTTTAACCCCAAATCTATGAGCTTCTTTGATGCATTCTTATCTCGAAGTAA
ACCCGGTTGAGTCTCTCTCGGAAATCCGGGAAACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATC
CG
   
Sequence Name 02-K118300-0022-581-G09C-oriL
GenBank Accession LN487233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118300-0022-581-G09C-oriL
GATTCGAGAGAATCACCGGGTTACTTCGAGATAAGAATGCATCAAAGAAGCTCATAGATT
TGGGGTTAAAGTTCATTTCCATGGAGGAAATCATCAAGGAAGGTGTGGAGAGTCTTAAAA
GCAAAGGATTTATCTCTTAATACCTACCTCTGTAACTCTGTTTGATTGAGCAATTAACAC
AATCTCTAGGCAGCAGAAACATTTCATATGATATACGATTTTGCACTTTTGTAATAAATA
AGCTGGCCTATGGTATGGTATGAACATCCAATTAATATTTATTAATCTGGCCTCTCTGAG
ATTTTTCTTTAAAGTTTGAACATGAGAATATCTTTAAGTGTTTTAAGTGATTTAGTTTTG
TTTATATAAATCCAGTGTTGCAATTATCACGAAATGTCAATATGTCCGAGTGGTTAAGGA
GACAGACTCGAAATCTGTTGGGCTTTGCCTGCGCAGGTTCGAATCCTGCTGTTGACGTTC
CTAATTAAATCGTTTTTTTCAATGTACCATTTTTCTTTTGTTTGGTAATAATATGTCCGC
GATTAATAATCAGTCTATTGGACTATTGGTTTACCAAGAGAATTAATGCTAGGTTGGTCC
TAAGTCCTTT
Primers used for PCR NG26 - 8474, show primer details
   
Sequence Name 01-K118300-0022-581-G09C-8409
GenBank Accession LN486777 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118300-0022-581-G09C-8409
ATTTACAATTGAATATTCAATTGAATAATTGGTAACCTAAGAGATTTTTCCAATTCAAGT
TATTGAAATCTCTCTCTCTATATATAACGTCTGTTTCAAAGAAAAATTTGTGGCTTACTT
GGGGACATTGTAATTGGGATAGAGCTCAGCAACTTTGGCTACAAAATCACCGTAGTGAGA
GATAGCCTCAACGCACAAGTGCCTTCCTTTCGAATATGGATCCTCGTATACAAGAATATG
AGCTAAAGCCACATCCTTGAAATGCACAGACCCCATAAAGAAGTTCTCGTATGTCTCCGT
GCACCCTAAGCATCCATATCTATTCAAATTCAAAGATGTTCCATGGAACTCAAGACATAT
TCAATCAAACATTCATAGAGAACCAAAATACCCTGAAGAAGGCGTAGAAGCATGTGCATG
CTAGCGTTAAGAGACGGAGGAATCACAGGCCCCATGACAGTGCCTGGATTCACCACAACC
ACATCCAATCCTTTCTCCTCTGCAAATTCCCAAGCTGCCTTCTCAGCAAGCGTCTTCGAC
AGTGGATACCACAACTACCAACAACAACACATCATCTATAAAGATAACCAATAAGATGTT
TTGCAGTAACAAATCTTAAATTAGAGAATAACATACTCCATTT

Last Updated on 06.12.2018 16:11