Sequences from confirmation amplicon


Confirmation sequences for line 582E04 and hit At1g50700 are shown.
Line ID 582E04
Locus At1g50700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18783982
Primers used for PCR TR12 - 8474, show primer details
   
Sequence Name 39-K027640-022-582-E04C-8409
GenBank Accession LN503232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K027640-022-582-E04C-8409
CCATTTACAATATATCCGGACATGAGAACGTTTACAAAGCCTTCCAACATTTTGACAAAG
ATGGGAGTGGGTAAGTGATCTTTCTTTTGGTATGAATCATTTTGCAATGTAAATAGATTT
GTTAGCAATGATTGATGAGACTATTACTGAAAATGTGTTTAATAGATACATTACGACAGA
CGAGCTAGAGGCTGCTTTGAAGGAATATGGAATGGGAGATGATGCTACTATCAAAGAGAT
TTTGTCTGATGTCGACGCTGATAATGTAAAAGCCAAAAGATTAAAGCTCTTCGCCCTTGT
AACGTTTTTCGTTTTCTTTCTCTGACCTCAAATTTTGTTTCTTGTTACAGGACGGTAGAA
TCAACTATGACGAGTTTTGTGCTATGATGAGAAGTGGGAATCCACAACAACCAAGATTGT
TCTAACATATTTTATTTGTCTTCTGTCTGAATCTAATCAAAAAAGACAAGAGTGGTATTA
TTATTGTTGTTTAGGCAAAAATTTAAGGTTGAAAAGAGAGAGAGAGAGATGATTGCTTTC
ATTTATTTTGGTACTGCTAAACTGGTCATCTCATGTGGTTT
   
Sequence Name 40-K027640-022-582-E04C-TR12
GenBank Accession LN503585 [GenBank]
Sequencing primer gene specific primer, TR12: 5'-CTTTTATTGGTCCTTCAAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K027640-022-582-E04C-TR12
ACATACAATATGAAGCATCATCTCTCTCTCTCTCTTTTCACCTTAAATTTTTGCCTAAAC
AACAATAATAATACCACTCTTGTCTTTTTTGATTAGATTCAGACAGAAGACAAATAAAAT
ATGTTAGAACAATCTTGGTTGTTGTGGATTCCCACTTCTCATCATAGCACAAAACTCGTC
ATAGTTGATTCTACCGTCCTGTAACAAGAAACAAAATTTGAGGTCAGAGAAAGAAAACGA
AAAACGTTACAAGGGCGAAGAGCTTTAATCTTTTGGCTTTTACATTATCAGCGTCGACAT
CAGACAAAATCTCTTTGATAGTAGCATCATCTCCCATTCCATATTCCTTCAAAGCAGCCT
CTAGCTCGTCTGTCGTAATGTATCTATTAAACACATTTTCAGTAATAGTCTCATCAATCA
TTGCTAACAAATCTATTTACATTGCAAAATGATTCATACCAAAAGAAAGATCACTTACCC
ACTCCCATCTTTGTCAAAATGTTGGAAGGCTTTGTAAACGTTCTCATGTCCGGATATATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11