Sequences from confirmation amplicon


Confirmation sequences for line 582F05 and hit At1g76670 are shown.
Line ID 582F05
Locus At1g76670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28773440
Primers used for PCR RU68 - 8474, show primer details
   
Sequence Name 31-K027064-022-582-F05C-8409
GenBank Accession LN500013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K027064-022-582-F05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGACTGCACCAATCCAAGCC
ATTTCACTTCTCATCTGTGGCCCCTTTGTTGACTATTTGTTGAGCGGCAAATTCATATCT
ACTTACCAGATGACTTATGGTGCCATCGTAAGTAAATTCTCAAGTTCTCTATGATTCAGA
TGACTCAAACGTAAACACAAAATCTTTACTTTTTATCTCTCATTTTTGCAGTTCTGCATT
CTTCTCTCCTGTGCATTAGCCGTTTTCTGTAACATAAGCCAATATCTCTGCATCGGAAGA
TTCTCAGCGACATCTTTCCAAGTTCTAGGCCACATGAAAACAGTATGCGTCCTCACATTA
GGATGGCTTCTCTTTGATTCCGAGATGACATTCAAGAACATCGCCGGGATGGCCATAGCC
ATTGTTGGAATGGTGATCTATAGTTGGGCTGTTGATATAGAGAAACAGAGGAACGCAAAG
TCAACGCCACACGGCAAACACAGTATGACCGAAGATGAGATTAAGCTACTCAAAGAAGGA
GTTGAGCATATCGACTTGAAAGACGTTGAACTCGGTGACACTAAACCATAAGTGGGTGCT
TATAACCCATTTGAAATTACTCGTCGCTACTGATTCGAATTCTTTATACAAATATTTCCT
TTGTGTGCCATTTACTTTGTTCTTTCCACTTCCTATAT
   
Sequence Name 32-K027064-022-582-F05C-RU68
GenBank Accession LN500435 [GenBank]
Sequencing primer gene specific primer, RU68: 5'-CTGAAAAGCCCAAAATGTTCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K027064-022-582-F05C-RU68
ATATATAGGAAGTGGAAAGAACAAAGTAAATGGCACACAAAGGAAATATTTGTATAAAGA
ATTCGAATCAGTAGCGACGAGTAATTTCAAATGGGTTATAAGCACCCACTTATGGTTTAG
TGTCACCGAGTTCAACGTCTTTCAAGTCGATATGCTCAACTCCTTCTTTGAGTAGCTTAA
TCTCATCTTCGGTCATACTGTGTTTGCCGTGTGGCGTTGACTTTGCGTTCCTCTGTTTCT
CTATATCAACAGCCCAACTATAGATCACCATTCCAACAATGGCTATGGCCATCCCGGCGA
TGTTCTTGAATGTCATCTCGGAATCAAAGAGAAGCCATCCTAATGTGAGGACGCATACTG
TTTTCATGTGGCCTAGAACTTGGAAAGATGTCGCTGAGAATCTTCCGATGCAGAGATATT
GGCTTATGTTACAGAAAACGGCTAATGCACAGGAGAGAAGAATGCAGAACTGCAAAAATG
AGAGATAAAAAGTAAAGATTTTGTGTTTACGTTTGAGTCATCTGAATCATAGAGAACTTG
AGAATTTACTTACGATGGCACCATAAGTCATCTGGTAAGTAGATATGAATTTGCCGCTCA
ACAAATAGTCAACAAAGGGGCCACAGATGAGAAGTGAAATGGCTTGGATTGGTGCAGTCA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
Primers used for PCR RU69 - 8474, show primer details
   
Sequence Name 43-K027065-022-582-F05C-8409
GenBank Accession LN504712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K027065-022-582-F05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACATGAATTGCTCAGCAATTCAAAGGATCCTAC
TGAGTACTTCTTCTGCAGAGAGCCTATAGACTGTAACCAAAAGAAAAAGTGAAAATTTGT
CAGCAGAACACTTGACACGCCATAGTATCTAAGAAGTTTCGAGAGAAAAATGCCCTTTCC
AGGACTCCAGCAAGGAATACAGAACTGTGTCTCCTAGATTTATAATTTCGAATTCAGATC
TGGAAAGGAAATCTAGTTGAGTTTACGTTATGCAGGAGAATCTGTGTACAATACTGAGTT
AGATTAGACATAGATGGAGCTCAAAGGGACAAAAGATTCCCTCCAAATACCACTGATCTA
AGAAAAGTTTGAGCCTTTCTGTTTCAAAGAATGTAAAAAGATGAATACTGATCTAAGAAA
TTCCAGATCTAACATAAACAAAGAGATGGTGTGATGAATACTTACAATCTGCTGCAGAGA
AGTGGAGAAAACGGCAGTGCATGCACAAATGAAACC
   
Sequence Name 44-K027065-022-582-F05C-RU69
GenBank Accession LN505056 [GenBank]
Sequencing primer gene specific primer, RU69: 5'-GTTAATGCCAAAGGTTTCATTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K027065-022-582-F05C-RU69
TTTTCTCCACTTCTCTGCAGCAGATTGTAAGTATTCATCACACCATCTCTTTGTTTATGT
TAGATCTGGAATTTCTTAGATCAGTATTCATCTTTTTACATTCTTTGAAACAGAAAGGCT
CAAACTTTTCTTAGATCAGTGGTATTTGGAGGGAATCTTTTGTCCCTTTGAGCTCCATCT
ATGTCTAATCTAACTCAGTATTGTACACAGATTCTCCTGCATAACGTAAACTCAACTAGA
TTTCCTTTCCAGATCTGAATTCGAAATTATAAATCTAGGAGACACAGTTCTGTATTCCTT
GCTGGAGTCCTGGAAAGGGCATTTTTCTCTCGAAACTTCTTAGATACTATGGCGTGTCAA
GTGTTCTGCTGACAAATTTTCACTTTTTCTTTTGGTTACAGTCTATAGGCTCTCTGCAGA
AGAAGTACTCAGTAGGATCCTTTGAATTGCTGAGCAATTCATGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGA

Last Updated on 06.12.2018 16:11