Sequences from confirmation amplicon


Confirmation sequences for line 584F02 and hit At1g20640 are shown.
Line ID 584F02
Locus At1g20640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7157246
Primers used for PCR 21wu - 8474, show primer details
   
Sequence Name 43-K118761-0022-584-F02C-21wu
GenBank Accession LN505009 [GenBank]
Sequencing primer gene specific primer, 21wu: 5'-GCTCATATCCTCTATGCTAAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118761-0022-584-F02C-21wu
GCGAATTCCGCATCCGTAATCTGATCTTCTCTTCTCCGTAGCTAACTTTAATCCTCAGAA
AGTCATCATCTTGAGATGATAACAAGTTGGTGCCTTGAGGGATGTTTTCTAAACTGGAGA
GAGTCAAAATTGTCTCGTCAAGACTCGAGCTTTGAAGCTCGATTTCGCTGCTCGTCTTCT
TCAATGCACCTCCTACATCAGTTGAAGGAGGATCGGTTGTTGCACCGCTGTTTAGTTGGG
TTTCACTGGAGCAACATTGGCTAGAGTTTGAACTGTGACTATACGAGGATACAGGGGACT
TTGCAAGCTGCACTGGCGGCGGAGGAGGAGGCGTGGTCTTGGCTTGTTGTGATGGTTCTT
GTGACTGTGAGACTAAATTGGGGAAATTTGCATAGAATGAGCCTATGGGAAGAGGACCAG
AAACACCTTGAACCGAATCAATCACTCGTTGGATCTTCTGCAGAGAATGTCCCACTTTTT
TTATCTTTCTTGAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 18-K118760-0022-584-F02C-8409
GenBank Accession LN494524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118760-0022-584-F02C-8409
AATTGAATATATCCTTCAAGAAAGATAAAAAAAGTGGGACATTCTCTGCAGAAGATCCAA
CGAGTGATTGATTCGGTTCAAGGTGTTTCTGGTCCTCTTCCCATAGGCTCATTCTATGCA
AATTTCCCCAATTTAGTCTCACAGTCACAAGAACCATCACAACAAGCCAAGACCACGCCT
CCTCCTCCGCCGCCAGTGCAGCTTGCAAAGTCCCCTGTATCCTCGTATAGTCACAGTTCA
AACTCTAGCCAATGTTGCTCCAGTGAAACCCAACTAAACAGCGGTGCAACAACCGATCCT
CCTTCAACTGATGTAGGAGGTGCATTGAAGAAGACGAGCAGCGAAATCGAGCTTCAAAGC
TCGAGTCTTGACGAGACAATTTTGACTCTCTCCAGTTTAGAAAACATCCCTCAAGGCACC
AACTTGTTATCATCTCAAGATGATGACTTTCTGAGGATTAAAGTTAGCTACGGAGAAGAG
AAGATCAGATTACGGATGCGGAATTCGCGCAGGTTAAGAGATCTATTGTGGGAGATTGGG
AAGCGGTTTAGCATAGAGAATATGAGC

Last Updated on 06.12.2018 16:11