Sequences from confirmation amplicon


Confirmation sequences for line 584H09 and hit At1g68260 are shown.
Line ID 584H09
Locus At1g68260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25587288
Primers used for PCR NG25 - 8474, show primer details
   
Sequence Name 09-K102362-0022-584-H09C-8409
GenBank Accession LN490120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K102362-0022-584-H09C-8409
TTGCGCAATTTTCAAAGTTTTTTGAATCACGGTTTATTTTGTAGAGTTCAACTCAAAGTT
GAGGTTGAACCAAACTACATTTCAAGTAGTTTTTTGAAGTTGATTTGACCATCTAACATC
AAATCATTTCAAGTTGAGGTTGAACTCAAAGTTCGTGATTATGAATTGGATCAGTTTGGT
GTTGTGAACAATGCTGTTTACGCAAACTACTGTCAACACGGTTAGTTTTCAATGTTGCTG
CTTCTGATTGTTGAAAGTGTTCATACATTTGTGAATTTAGTTGATAAAATCTGAACTCTG
CATGATCAAAGTTACTTCTTTACTTAGTTTGACAGGGACTTTTTTTGTGAATGTGGTTGA
GTAGAATTTAGGGCTTTGGATTAAATGTGACAAGATTTTGCTAGTAAGTTTAGAGTCTTG
CTAGTAATTCAGTTAGTTTGTGATGTTGCTGCTTCAGATTGTGTACTTTTCTATTGTTAA
AGAGGAAGTTTCAGATCGTGTTCTTGACAGAATCTGGAATGCTTGATCAATAGTTACTCC
TTTTCTGAGTCTAATTGGACAATTTTTTGAATGTGGCTGACTAGAAATTGAGGTGATGTT
GCAGNTAATTC
   
Sequence Name 09-K102363-0022-584-H09C-NG25
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NG25: 5'-CGAATTACTAGCAACATCACCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K102363-0022-584-H09C-NG25
ACCTTCGAAAAAAGGAAATCTGAGTTTTGGCC

Last Updated on 06.12.2018 16:11