Sequences from confirmation amplicon


Confirmation sequences for line 585A09 and hit At2g40410 are shown.
Line ID 585A09
Locus At2g40410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16873691
Primers used for PCR 38aq - 8474, show primer details
   
Sequence Name 07-K106771-0022-585-A09C-8409
GenBank Accession LN489290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106771-0022-585-A09C-8409
ATTTACATTGAATATATCCAGTGATCTTTAAGCTAATTCAGCTGTTGGAAGGTCTCTTTA
CTTGATTTGGTTCAAAAGTTGATTTTGATGATGAGTTTACCTGTAGGTTCCTGAAGGGCT
TGGGAGGTATGTTCAGTCATCTAGAAAAGCTCAAGCAAACTGGTAAAATAACACAAGATT
GCTTTACATGTTTCGTGTAGGATAAAGATGTGAACTTTGTTGTGTAATCAATCTGTTTGT
CATGTATTTTAACATTGTGAAAGGTATAGAAAAATACTTGAGGCATGGAAGCAAGCAAAA
CCTCCACCGCAGACAGCTGAGGAAGCTTCTAGGCTTGTCACTGATATCTTAAAAAGGAAT
CAGAAAGCTGATGTTGAGGTATGATTAGC
   
Sequence Name 23-K106772-0022-585-A09C-38aq
GenBank Accession LN496574 [GenBank]
Sequencing primer gene specific primer, 38aq: 5'-TGCTAATCATACCTCAACATCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106772-0022-585-A09C-38aq
GCCTAGAAGCTTCCTCAGCTGTCTGCGGTGGAGGTTTTGCTTGCTTCCATGCCTCAAGTA
TTTTTCTATACCTTTCACAATGTTAAAATACATGACAAACAGATTGATTACACAACAAAG
TTCACATCTTTATCCTACACGAAACATGTAAAGCAATCTTGTGTTATTTTACCAGTTTGC
TTGAGCTTTTCTAGATGACTGAACATACCTCCCAAGCCCTTCAGGAACCTACAGGTAAAC
TCATCATCAAAATCAACTTTTGAACCAAATCAAGTAAAGAGACCTTCCAACAGCTGAATT
AGCTTAAAGATCACTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCACACGGTTATT
Primers used for PCR 01bg - 8474, show primer details
   
Sequence Name 64-K118471-0022-585-A09C-01bg
GenBank Accession LN511296 [GenBank]
Sequencing primer gene specific primer, 01bg: 5'-TGGACCACCTACAATTGTGATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118471-0022-585-A09C-01bg
ATGTAAAAATTGTTGCCTTATCTTAATATTCTCCAACTTATTTTTTCTTCTCCTTTTAAA
TTAAATCATTTTTATTTATTTATTGAAAAGTTAATTACAGATAAGAGTAAGTGTTTGAGA
GTTGACTCCTTTGTCCTTCCTCTTTAGGATTCTCCTCTGGGCTCTGGTAAGGTATTAGAA
AGATCAAAACTTTTTGGATTCCCTGAAATTGGGTGAAAGATGGGTAACGCTCTTACGTTT
CTCTACGGAAAATGCTGCAAACCCACAACAACCGATGACTCTCTTGGTCCTCACGGCGTC
TCCGCCGCCACCGTCGGTGTTTCAGCTCTCGCTCACGATCTCTTCAACTTCGAGATCACT
TCCCAGGTCTTAATTTTCTCACTGAAACTTCTAACCGTCCTTTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTTATTATAAA
   
Sequence Name 32-K118470-0022-585-A09C-8409
GenBank Accession LN500766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118470-0022-585-A09C-8409
CATTTCAAGGACGGTTAGAAGTTTCAGTGAGAAAATTAAGACCTGGGAAGTGATCTCGAA
GTTGAAGAGATCGTGAGCGAGAGCTGAAACACCGACGGTGGCGGCGGAGACGCCGTGAGG
ACCAAGAGAGTCATCGGTTGTTGTGGGTTTGCAGCATTTTCCGTAGAGAAACGTAAGAGC
GTTACCCATCTTTCACCCAATTTCAGGGAATCCAAAAAGTTTTGATCTTTCTAATACCTT
ACCAGAGCCCAGAGGAGAATCCTAAAGAGGAAGGACAAAGGAGTCAACTCTCAAACACTT
ACTCTTATCTGTAATTAACTTTTCAATAAATAAATAAAAATGATTTAATTTAAAAGGAGA
AGAAAAAATAAGTTGGGAGAATAATTAAGAATAAGGCAACAATTTTTTTTTTACAATTCT
CTTTGTTAAATAAATCACAATTGTAGGGTTGGTCCAA

Last Updated on 06.12.2018 16:11