Sequences from confirmation amplicon


Confirmation sequences for line 585B02 and hit At2g36100 are shown.
Line ID 585B02
Locus At2g36100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15159717
Primers used for PCR AH44 - 8474, show primer details
   
Sequence Name 54-K105395-0022-585-B02C-AH44
GenBank Accession LN508477 [GenBank]
Sequencing primer gene specific primer, AH44: 5'-AGTAAGACAAGTCATGTTCCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K105395-0022-585-B02C-AH44
CTCGTTTTTTGAAGGTACTTTGTGATAGCCGTAGCCGTAGTCGCTAGCTATCTCGTCCTT
TCACTTCCATTCTCCATCGTATCCATTGTCCGTCCACATGCTGTCGCGCCCCGGCTGATC
CTCCTCATTTGCGATACTGTAACTTACCTTATAACTCTATTCTTCATTTCTTTCATAATT
TGATATAACTCACACTCTTTTATGTTATGATTGTATATATAAAGCTGGTCGTGACGCTCA
ACACATCAGCAGCAGCAGCGGCAGCATCAATCACCTACCTTGCACACAACGGCAACCAAA
GCACCAACTGGCTCCCTATCTGTCAGCAGTTTGGAGACTTCTGCCAGAACGTTAGCACCG
CGGTTGTGGCTGATTCTATCGCGATTCTCTTCTTCATCGTTCTTATCATCATCTCAGCCA
TCGCCCTCAAGAGGCATTGATTTCATCTTTAAGTGTCAAGATCGTAATGTACTTGTTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 38-K105396-0022-585-B02C-8409
GenBank Accession LN502977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105396-0022-585-B02C-8409
TTACGATCTTGACACTTAAAGATGAAATCAATGCCTCTTGAGGGCGATGGCTGAGATGAT
GATAAGAACGATGAAGAAGAGAATCGCGATAGAATCAGCCACAACCGCGGTGCTAACGTT
CTGGCAGAAGTCTCCAAACTGCTGACAGATAGGGAGCCAGTTGGTGCTTTGGTTGCCGTT
GTGTGCAAGGTAGGTGATTGATGCTGCCGCTGCTGCTGCTGATGTGTTGAGCGTCACGAC
CAGCTTTATATATACAATCATAACATAAAAGAGTGTGAGTTATATCAAATTATGAAAGAA
ATGAAGAATAGAGTTATAAGGTAAGTTACAGTATCGCAAATGAGGAGGATCAGCCGGGGC
GCGACAGCATGTGGACGGACAATGGATACGATGGAGAATGGAAGTGAAAGGACGAGATAG
CTAGCGACTACGGCTACGGCTATCACAAAGTACCTTCAAAAAACGAGTATATTATTTCAT
TAGGTTACTAACCGCACAATGATATAACCGGAACATGACTGGGTCTTAC

Last Updated on 06.12.2018 16:11