Sequences from confirmation amplicon


Confirmation sequences for line 585B06 and hit At2g26610 are shown.
Line ID 585B06
Locus At2g26610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11321025
Primers used for PCR NU68 - 8474, show primer details
   
Sequence Name 33-K104096-0022-585-B06C-NU68
GenBank Accession LN500971 [GenBank]
Sequencing primer gene specific primer, NU68: 5'-TGAACACTAGAAATGTTGCTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104096-0022-585-B06C-NU68
GGTGGGTATGTTAATAAGCTCGTCGTCACATGTCTTCGGAGTGGCGTCAGTAGAGGCTTT
CGAGTATTGCAAAAGCCAGAGCGAGCTCCTATAAGAGCTTTGAGGGCTTCATCTTCTGGG
AGGTTGGTTTCTTTACTTTTTCATAGGCTTCACAAAGAGATGAAAGAGTCTTTGACATCA
TGTTTTCAGGTATCTTCTTATTTTGTTCCGTGATGCTCCAGTAGAAGTATGGGCAATGAC
TAAAAGCCCTGTGATGGTGAGTTTGGAGTGATGGAAATACTTGAGAAAGTTATGATGAAA
TGGGTTTGTAGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 49-K104097-0022-585-B06C-8409
GenBank Accession LN506822 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104097-0022-585-B06C-8409
GAAGCCATTTACAATTGAATATAACTACAAACCCATTTCATCATAACTTTCTCAAGTATT
TCCATCACTCCAAACTCACCATCACAGGGCTTTTAGTCATTGCCCATACTTCTACTGGAG
CATCACGGAACAAAATAAGAAGATACCTGAAAACATGATGTCAAAGACTCTTTCATCTCT
TTGTGAAGCCTATGAAAAAGTAAAGAAACCAACCTCCCAGAAGATGAAGCCCTCAAAGCT
CTTATAGGAGCTCGCTCTGGCTTTTGCAATACTCGAAAGCCTCTACTGACGCCACTCCGA
AGACATGTGACGACGAGCTTATTAACATACCCACCTGTTCGTTTACTCACCTGACATCAC
CAAAGCAACATTTCTNGGTGTTC

Last Updated on 06.12.2018 16:11