Sequences from confirmation amplicon


Confirmation sequences for line 585C12 and hit At4g20370 are shown.
Line ID 585C12
Locus At4g20370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11002318
Primers used for PCR HM47 - 8474, show primer details
   
Sequence Name 87-K033099-022-585-C12C-8409
GenBank Accession LN514712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K033099-022-585-C12C-8409
CATTTACATTGAATATATAGTAACATTTTCCATTAATATAAGGGTATCTAAAAATACTGG
CTTTACTATTCAGATTCAAAAGTCGTTAAAATAAGTGTCAATTAAGATATTTATGCTTTT
CTAAAGGTATGTATCCTAGAATAAAAAAACTAAAATAAAAATAAAAGCGAAACAAAATCA
AGTTAACCGTCTCTTGAAAACGTAATATATATAATCGAGAAAGATTCAAAATTGATTTAA
AATAATAATCAAACCAATAACAGTTATGCATGCAAGAAGTTAGAGGAAACGCAGCAGGTA
TATAGATGAGGCTACGAAAGAAAAGAATGATCAAATCTAGTTACGCTTAAATTTGGATAT
ATCTAAGGTTTCTTCGTCAATATATGGAGCTTACGAAAACGAAAGAGTGAGCTACGAGGA
ACTAAATCAATGAAGATAAGAGGAATGAAGGAGAGAA
   
Sequence Name 39-K033099-022-585-C12C-HM47
GenBank Accession LN503261 [GenBank]
Sequencing primer gene specific primer, HM47: 5'-CTACACCTTGGTGATCTTCTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033099-022-585-C12C-HM47
TCACTCTTTCGTTTTCGTAAGCTCCATATATTGACGAAGAAACCTTAGATATATCCAAAT
TTAAGCGTAACTAGATTTGATCATTCTTTTCTTTCGTAGCCTCATCTATATACCTGCTGC
GTTTCCTCTAACTTCTTGCATGCATAACTGTTATTGGTTTGATTATTATTTTAAATCAAT
TTTGAATCTTTCTCGATTATATATATTACGTTTTCAAGAGACGGTTAACTTGATTTTGTT
TCGCTTTTATTTTTATTTTAGTTTTTTTATTCTAGGATACATACCTTTAGAAAAGCATAA
ATATCTTAATTGACACTTATTTTAACGACTTTTGAATCTGAATAGTAAAGCCAGTATTTT
TAGATACCCTTATATTAATGGAAAATGTTACTATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11