Sequences from confirmation amplicon


Confirmation sequences for line 585G01 and hit At3g46950 are shown.
Line ID 585G01
Locus At3g46950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17290343
Primers used for PCR KU70 - 8474, show primer details
   
Sequence Name 24-K103311x-0022-585-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K103311x-0022-585-G01C-8409
GGAATGAAGCCATTTACAATTGAATATATCCTGTGGTTTGGTAGAGGAAGAGATTATTTC
GGTGTTGAAGAGTCGTCCACAATGCATACGATCTTCTGAGCAGAAGATATTAGACTCTAT
TGAAATGTTCTTAGGCTTAGGATTCAGTAGAGATGATTTCAAGATGATGGTTAAGCGATA
TCCTTGTTGCACTGCATATTCTGGCGAGACGCTGAGGAAGAAGTTTGAGGTTTTGGTGAA
GATGATGAATTGGCCGCTAGAGGCTGTGGTCATGATCCCTACGGTACTTGGATACAGCTT
GGAGAAGAGGATAGTGCCAAGGTCTAACGTGATCAAAGCTCTCATGTCGAAAGGATTGAG
CGGAAGTGAAAACCCTCCGATTTCATCTGTCTTGGTATGTACTGATCAGGAGTTCTTGAA
AAGGTATGTGATGAAGCATGACAAGCTAGTGCCTAAGTTGATGGCTATCT
   
Sequence Name 19-K103312x-0022-585-G01C-KU70
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KU70: 5'-GAAGATAGCCATCAACTTAGGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103312x-0022-585-G01C-KU70
CACATACCTCTTTCAGAACTCCTGATCAGTACATACCAAGACAGATGAAATCGGAGGGTT
TTCACTTCCGATCAATCCTTTCGACATGAGAGCTTTGATCACGTTAGACCTTGGCACTAT
CCTCTTCTCCAAGCTGTATCCAAGTACCGTAGGGATCATGACCACAGCCTCTAGCGGCCA
ATTCATCATCTTCACCAAAACCTCAAACTTCTTCCTCAGCGTCTCGCCAGAATATGCAGT
GCAACAAGGATATCGCTTAACCATCATCTTGAAATCATCTCTACTGAATCCTAAGCCTAA
GAACATTTCAATAGAGTCTAATATCTTCTGCTCAGAAGATCGTATGCATTGTGGACGACT
CTTCAACACCGAAATAATCTCTTCCTCTACCAAACCACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11