Sequences from confirmation amplicon


Confirmation sequences for line 586B09 and hit At5g35580 are shown.
Line ID 586B09
Locus At5g35580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13763494
Primers used for PCR NG22 - 8474, show primer details
   
Sequence Name 10-K102362-0022-586-B09C-8409
GenBank Accession LN490597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K102362-0022-586-B09C-8409
ATTTACAATTGAATATCCCAATGGGAATATCATCTTTGTAATCTTTGATGTCTTGGAGAA
CGGAGACAACAGTGCTAATGTCTGGCCTTGTCTTTGGTCGGTACCTTAGGCATTGATAAG
CTAGAGTTGCTGCCTTTCGTGCTCCGGTCTCTGAGTACTGATCTTCAAGCCTCGGGTCCA
TTATTCTCCCGAGTTTACGTGCGTCGTTGAGCATTGGACGAGCCCACTCAACGAGGGTTT
CTTTTCTTGATGATCTCGCTATGTCCACCGATTTCCGACCTGTCAACAGCTCAAGCAACA
CCACTCCAAAGCTGTACACATCACTCTTAGCCGTTAAATGACCTGTCATTATGTACTCTG
GCGCCGCGTACCCTTGTGTGCCCATCACGCGAGTCGACACATGTGTATCATCGCCTTGTG
GTCCGTCTTTTGCTAGCCCGAAGTCCGAGAGTTACCTGGTATAAT
Primers used for PCR NG23 - 8474, show primer details
   
Sequence Name 11-K102362-0022-586-B09C-OriL
GenBank Accession LN491056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K102362-0022-586-B09C-OriL
ATTTACATTGAATATATCCTGTCATGAAGCCATTTACGCCGGGAAGTTAAAGAGACAAGT
CTTCAAAACTTTGACAAGCCACGCCGTGAAACTAAAGTGACAAGTCTTCAAAACTTCGAC
AAAACACGCCGTGAAGTTAAAGATACAAGTCTTCAAAACTTCGACAAAACACGCCGTGAA
GTTAAAGAGACAAGTCTTCAAAACTTCGACAAAACACGCCGTGAAGTTAAAGAGACAAGT
CTTCAAAACTTCGACAAGCCTCGCAACGTTTCCACGACTGATAACCATCAAAAGTTCCGG
TCGCCCGCACACACGGCAAGAAACCACCGGATAACTTTGAGAAACGGATATAATTCACCA
ATGCA
   
Sequence Name 11-K102363-0022-586-B09C-NG23
GenBank Accession LN491057 [GenBank]
Sequencing primer gene specific primer, NG23: 5'-GCATTGGTGAATTATATCCGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K102363-0022-586-B09C-NG23
GGGCGACCGGAACTTTTGATGGTTATCAGTCGTGGAAACGTTGCGAGGCTTGTCGAAGTT
TTGAAGACTTGTCTCTTTAACTTCACGGCGTGTTTTGTCGAAGTTTTGAAGACTTGTCTC
TTTAACTTCACGGCGTGTTTTGTCGAAGTTTTGAAGACTTGTATCTTTAACTTCACGGCG
TGTTTTGTCGAAGTTTTGAAGACTTGTCACTTTAGTTTCACGGCGTGGCTTGTCAAAGTT
TTGAAGACTTGTCTCTTTAACTTCCCGGCGTAAATGGCTTCATGACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGTATAGTCAAT
ATA

Last Updated on 06.12.2018 16:11