Sequences from confirmation amplicon


Confirmation sequences for line 586H03 and hit At2g01510 are shown.
Line ID 586H03
Locus At2g01510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:232498
Primers used for PCR AH25 - 8474, show primer details
   
Sequence Name 56-K105395-0022-586-H03C-AH25
GenBank Accession LN509100 [GenBank]
Sequencing primer gene specific primer, AH25: 5'-TTCCAAGCCACTAAATCTTTGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K105395-0022-586-H03C-AH25
CCGAACTCATTCACCAAACTTCATGTACATCATTACCAACTCAGTTGCAACAATCCCAAG
ACACCCAAAACCATATTTCACCACATGGGCATGAAGAGCAAACCCACAGGAAAAATCCCC
AAGCTGAGAGATCGCTTTCACCACAAACGGGTAAGTAAATTCATCAGGACGGACACCAAG
ATCACGCATTTTCTTATAAAGTAGGAGACTTTCAAAAGGGAGCTGGTTTCTAACGTACCC
TTTGAAAAGAGTGTTCCAAAGGAAGATCCTCGGCTTGTGCATTTCGTCGAACACCTGGCG
TGCGTAACACATGTCACCAATAACTACAAGATTCTCTAACAGCTGAGTTAAAAGACTGTT
CTTTTCAGAGAAACCTGTCCTTAGTACAATCGCATGGATCTTCTTAAGCTGCTTCGGCTT
TGAAGAACTTGCTCGTAAGAGCTCGGAGAGCATTTGTTTTGTTAATGGCGTTTGTTTCAT
TGCTGATCCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 40-K105396-0022-586-H03C-8409
GenBank Accession LN503706 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K105396-0022-586-H03C-8409
ATTTCATTGAAATGGATCAGCAATGAAACAAACGCCATTAACAAAACAAATGCTCTCCGA
GCTCTTACGAGCAAGTTCTTCAAAGCCGAAGCAGCTTAAGAAGATCCATGCGATTGTACT
AAGGACAGGTTTCTCTGAAAAGAACAGTCTTTTAACTCAGCTGTTAGAGAATCTTGTAGT
TATTGGTGACATGTGTTACGCACGCCAGGTGTTCGACGAAATGCACAAGCCGAGGATCTT
CCTTTGGAACACTCTTTTCAAAGGGTACGTTAGAAACCAGCTCCCTTTTGAAAGTCTCCT
ACTTTATAAGAAAATGCGTGATCTTGGTGTCCGTCCTGATGAATTTACTTACCCGTTTGT
GGTGAAAGCGATCTCTCAGCTTGGGGATTTTTCCTGTGGGTTTGCTCTTCATGCCCATGT
GGTGAAATATGGTTTTGGGTGTCTTGGGATTGTTGCAACTGAGTTGGTAATGATGTACAT
GAAGTTTGGTGAATTGAGTTCGGCTGAGTTTTTGTTTGAGTCAATGCAAGTCAAAGATTT
ATG

Last Updated on 06.12.2018 16:11