Sequences from confirmation amplicon


Confirmation sequences for line 588B07 and hit At1g64910 are shown.
Line ID 588B07
Locus At1g64910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24115713
Primers used for PCR 16kn - 8474, show primer details
   
Sequence Name 42-K113916-0022-588-B07C-16kn
GenBank Accession LN504538 [GenBank]
Sequencing primer gene specific primer, 16kn: 5'-CAAGATTCTCCAAAGTGTCAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113916-0022-588-B07C-16kn
ATTCGGGAAAGGGTTTCCTTTCTTCACCAGAATTTCCCGATCTTCACTAGCTTGGGTTCC
ATTCACAGAAGCGATAGCAACACTTCAAGCTTCTCCTTCGAGAACCATCCTGTTTTCTTT
CTCTTTGCACTTTCACCCGAAACCTTGAGTTCTTCGGTCATCAATCTTGTGTTCGAGAAC
TTCGATCAGCCAAAAATCGGAAGCAAAACTATTTGGCAATCACTCATTATAGATTCCCAC
ATTGACCCGAATCCGCAATGACTCAAGAAGCAGCCTACTGATGGATGAGCCAATAATAAC
GGTTGTTGCACCCATTCTCCCAAAACCTCTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 14-K113915-0022-588-B07C-8409
GenBank Accession LN492589 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113915-0022-588-B07C-8409
GTGCAACAACCGTTATTATTGGCTCATCCATCAGTAGGCTGCTTCTTGAGTCATTGCGGA
TTCGGGTCAACGTGGGAATCTATAATGAGCGATCGCCAAATAGTTTTGCTTCCATTTTTT
GGCTGATCAAGTTCTCAACACAAGATTGATGACCGAAGAACTCAAGGTTTCGGTTGAAGT
GCAAAGAGAAGAAACAGGATGGTTCTCGAAGGAGAGCTTGAGTGTTGCTATCACATCTGT
GATGGACCAAGCTAGTGAGATCGGGAATCTGGTGAGAAGGAACCATTCCAAATTGAAGGA
GGTTTTGGTTAGTGATGGATTATTAACCGGTTACACCGATTAATTTGTTGACCTT

Last Updated on 06.12.2018 16:11