Sequences from confirmation amplicon


Confirmation sequences for line 589A02 and hit At1g63250 are shown.
Line ID 589A02
Locus At1g63250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23466139
Primers used for PCR 19wm - 8474, show primer details
   
Sequence Name 84-K118585-0022-589-A02C-19wm
GenBank Accession LN514477 [GenBank]
Sequencing primer gene specific primer, 19wm: 5'-ATCAAATTCCTTTACACAGCCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118585-0022-589-A02C-19wm
GAGGCTCTTGCTTCTCCTCATTACATTATCCACAGATTTTCTTTTACTGAGATCTTCTCT
AACTGTATTAATATCCAAAGGAGGATAAAGCTCTTCATCCGTGACATTACGATTCACTCT
TTTCGTCAGCTTGATATCGTGGTTCCCTAACGACGCACTGCTTCCCGTTTTCCTCATATC
CATCCAACCTATCATCTTTCCCTCATCGCCTTCTTCATCTGAATCCTCTTCAGTTGAACT
ATCGTTCTTCCTAAACTTCCTTCTTCCAAACAAACCTTTGTTCCTTATAACTCCAACAAC
ACTCCTCGAGCTCTTCACTTTCTCCTTGGTACCAGCAAATTTAGGAGAAAAAGGACTCAA
AGTATCTAGCTTTTTAGCATCAAAGTCATCTCCTTCATCACAAGAACTCTCATTTCTCTT
AAATCTACTCATACTCGAAAAACCTCTCCTACTACTACCGAAAACACGATTTTTACCCAT
AGAATCACATGTCGCAGAAACTAATCTCCTCACATCAATAGGCGAATTAGAAGTAGAATC
AATCGAATCCGAACCTGATCTCGACCCTTGATCTGATTTCTTCACCGGACCATCGTTCTC
GTTCCAAAAATCCTCTGCTCCAGCTTTCATAAACCGATCAGCCAAGGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCCGTTATTAT
   
Sequence Name 54-K118584-0022-589-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118584-0022-589-A02C-8409
CTAAAANNAGCTAGATANNCTTTGAGNNTCCTTNNTTTCTCCNNTAAATTTGCTNNGGTA
CCAAGGNNNAGAAAGTGAAGAAGCNNTCGAGGAGTGGTNNTGTTGGANNGTTATAAGGGG
AACAAAGGGGTTTGTTTNNGGAAGAAGGNANNAGTTTAGGAAGAAACGATNNAGTTCAAC
TGAAGAAGGAATTCAGAAATGAAGAAGGCGNNNATGAGGGAANNAGATGATAGGTNNTGG
ATGGATATNNGAGGAAAACGNNGGAAGCAGTGGGCGTCGTTANNGGGAACCATNCGATAT
CAAGCTGAAAACGAAAAGAANGTGAATCGTAATGGTCACNNGGATGAAGAAGNNCTTTAT
CNNCTCCTTTGGNNNATATTAATACANNGTTAGAAGAAGAAATCTCNNAGTAAAAGAAAA
NNATCTGTGGNNNATAATGTANNATGGAGGAGNANNAGCAAGAGCNCNNTCATGACNNTC
GATTTACANGTGGGCAAAAAGGGGTATTTTANNAACTGGCNNTGTGTAAAG

Last Updated on 06.12.2018 16:11