Sequences from confirmation amplicon


Confirmation sequences for line 589H01 and hit At4g09760 are shown.
Line ID 589H01
Locus At4g09760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6150146
Primers used for PCR CH48 - 8474, show primer details
   
Sequence Name 10-K106998-0022-589-H01C-8409
GenBank Accession LN490678 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106998-0022-589-H01C-8409
CATTTACAATTGATGCAATGATCAACACAAAAATTTAACTACAGTATGAAGACAAATCAA
CAATAGGTGCACAGCTAAACCGGTTTGGTTTTTTCCGGTTTAACACCACATGAAAAAGGT
AAACCAATTATATGAGCTATAGCCTATAGGGATTGCTAAACCTAGTTTAACCCAATAAAA
AACTAAGCCGAAAGCTGAACCAAAGAAAATATAAGCCAATTCGGTTTCATTTAACTACAT
ATCCAAAAGAAAAATCAAACCGAACCGAATCGGAATTGATGAAAAACAAACAGTAAAATC
TGAAAATCATTTACAAATCAAAACCTGATGAATAATAATATCCGTCAAATATTTTGTAAC
TTGTAAGGAACCCAATTTATACCAAACATG
   
Sequence Name 26-K106999-0022-589-H01C-CH48
GenBank Accession LN497949 [GenBank]
Sequencing primer gene specific primer, CH48: 5'-CATGTTTGGTATAAATTGGGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106999-0022-589-H01C-CH48
TGAGGATATATATTCATCAGGTTTTGATTTGTAAATGATTTTCAGATTTTACTGTTTGTT
TTTCATCAATTCCGATTCGGTTCGGTTTGATTTTTCTTTTGGATATGTAGTTAAATGAAA
CCGAATTGGCTTATATTTTCTTTGGTTCAGCTTTCGGCTTAGTTTTTTATTGGGTTAAAC
TAGGTTTAGCAATCCCTATAGGCTATAGCTCATATAATTGGTTTACCTTTTTCATGTGGT
GTTAAACCGGAAAAAACCAAACCGGTTTAGCTGTGCACCTATTGTTGATTTGTCTTCATA
CTGTAGTTAAATTTTTGTGTTGATCATTGCATCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11