Sequences from confirmation amplicon


Confirmation sequences for line 590A12 and hit At1g22370 are shown.
Line ID 590A12
Locus At1g22370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7898833
Primers used for PCR MG38 - 8474, show primer details
   
Sequence Name 12-K032599-022-590-A12C-8409
GenBank Accession LN491463 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K032599-022-590-A12C-8409
TTGAATATATCCTACCTCAATCTATCATGCCTCAAGTGTACACTATTGGACCGCTTCATC
TATTTGTGAATCGGGATATCGACGAGGAAAGTGACATCGGACAGATAGGAACGAATATGT
GGAGAGAGGAGATGGAGTGTTTGGATTCGGCTTGATACTAAGTCTCCAAACAGTGTCGTT
TATGTTAATTTCGGTAGCATAACAGTGATGAGTGCGAAACAACTCGTGGAGTTTGCTTGG
GGTTTAGCAGCGACCAAAAAAGATTTTTTGTGGGTGATTAGGCCGGATTTAGTAGCCGGT
GATGTGCCAATGCTTCCGCCGGACTTTCTAATAGAGACGGCTAACCGAAGGATGCTAGCG
AGTTGGTGTCCTCAAGAAAA
   
Sequence Name 12-K032599-022-590-A12C-MG38
GenBank Accession LN491464 [GenBank]
Sequencing primer gene specific primer, MG38: 5'-AAGAACTTTTTCTTGAGGACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K032599-022-590-A12C-MG38
AAGTCCGGCGGAAGCATTGGCACATCACCGGCTACTAAATCCGGCCTAATCACCCACAAA
AAATCTTTTTTGGTCGCTGCTAAACCCCAAGCAAACTCCACGAGTTGTTTCGCACTCATC
ACTGTTATGCTACCGAAATTAACATAAACGACACTGTTTGGAGACTTAGTATCAAGCCAA
TCCAAACACTCCATCTCCTCTCTCCACATATTCGTTCCTATCTGTCCGATGTCACTTTCC
TCGTCGATATCCCGATTCACAAATAGATGAAGCGGTCCAATAGTGTACACTTGAGGCATG
ATAGATTGAGGTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11