Sequences from confirmation amplicon


Confirmation sequences for line 590C01 and hit At3g25020 are shown.
Line ID 590C01
Locus At3g25020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9117509
Primers used for PCR CH49 - 8474, show primer details
   
Sequence Name 48-K112250-0022-590-C01C-CH49
GenBank Accession LN506538 [GenBank]
Sequencing primer gene specific primer, CH49: 5'-TTTGAAACTCCGAAAGAACAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112250-0022-590-C01C-CH49
GCCTCTACGGNCACTCGACGTTGGCTACAATCGATTAACAGGGAAGCTGCCAAGGTCTCT
TATAAATTGCTCAGCTCTACAGTTTCTAAGTGTGGACCACAACGGAATCAAAGATACATT
TCCTTTCTCCCTCAAGGCTTTACCGAAATTGCAAGTCCTTCTCCTCAGTTCAAACAAATT
CTATGGTCCTCTATCTCCTCCTAATGAAGGTCCTCTGGGTTTTCCTGAGCTGCGGATACT
GGAGATAGCTGGTAACAAACTAACCGGAAGCTTGTCCTCAGACTTTTTTGTGAATTGGAA
AGCATCATCACACACGATGAATGAAGATCTGGGTCTATATATGGTATATGGCAAGGTTAT
TTTCGGGAACTATCACCTTACATAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 38-K112249-0022-590-C01C-8409
GenBank Accession LN503031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K112249-0022-590-C01C-8409
GGTGATAGTTCCCGAAAATAACCTTGCCATATACCATATATAGACCCAGATCTTCATTCA
TCGTGTGTGATGATGCTTTCCAATTCACAAAAAAGTCTGAGGACAAGCTTCCGGTTAGTT
TGTTACCAGCTATCTCCAGTATCCGCAGCTCAGGAAAACCCAGAGGACCTTCATTAGGAG
GAGATAGAGGACCATAGAATTTGTTTGAACTGAGGAGAAGGACTTGCAATTTCGGTAAAG
CCTTGAGGGAGAAAGGAAATGTATCTTTGATTCCGTTGTGGTCCACACTTAGAAACTGTA
GAGCTGAGCAATTTATAAGAGACCTTGGCAGCTTCCCTGTTAATCGATTGTAGCCAACGT
CGAGTGACCGTAGAGGCGTATCCTCATAATACTTGTCAGGAATACTTCCTTCCAAGTTGT
TCTTTC

Last Updated on 06.12.2018 16:11