Sequences from confirmation amplicon


Confirmation sequences for line 590E02 and hit At5g43695 are shown.
Line ID 590E02
Locus At5g43695 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17548426
Primers used for PCR 24lg - 8474, show primer details
   
Sequence Name 43-K113947-0022-590-E02C-24lg
GenBank Accession LN504912 [GenBank]
Sequencing primer gene specific primer, 24lg: 5'-CAAAGTCTTGCCACAATTCAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113947-0022-590-E02C-24lg
TTGCTTCCATATTTAAAACTATATGATCTATCTCTTGTAACTCTTAAACTGTTTGTTTTC
TAGTTTGCACTCTAGAATTGGTCTTATATTGTTAGTTTGTTACCGACTTTTTGTAGAATA
TCAGTTATACTAGTCTCATGTATATTTACAAAATTTTGTAATAAATTCATATAATTTTAC
TTAGTCAATAACTGGACTTTTTCCTTGTAATTTGATAAGTATTTATTTGGCTGGTAACAA
AAACAAAATAATTAGTAATTTACAGTAGATCCCTTTATATAACTATTGTATCTAGAAATA
GTTGTACTTCTACGTATTGGGGAGTACCCCGAGTTTTGTGTAACAACTTCCGCTATATAT
TGTAAAGTAAAAAAATACCAATGAAGTTTGCTTTCCTTATCTGCCTAATTGTTACATCAG
GTAAAATTTATATTTCACGTGTGAAGCAAATAACAAAAATCTATATACAAAATTTGTTAT
TTATAGTTTCTGATTTTGTAGTGATGAGTATCGTTACTGTTCGAAATGTTGAGGCGAAAA
GGTTATTACTTGAAGAAAGAGTAATGAGTAAATGGACATAAATACTGGCCGTTACTGTTT
GAAATGTTGGGAAGGTTTTCTCGTTACTTTTTCTTTTTCTTCATGATGTTCGAAATGTTT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 15-K113946-0022-590-E02C-8409
GenBank Accession LN493058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113946-0022-590-E02C-8409
TTGAAACATTTCGAACATCATGAAGAAAAAGAAAAAGTAACGAGAAAACCTTCCCAACAT
TTCAAACAGTAACGGCCAGTATTTATGTCCATTTACTCATTACTCTTTCTTCAAGTAATA
ACCTTTTCGCCTCAACATTTCGAACAGTAACGATACTCATCACTACAAAATCAGAAACTA
TAAATAACAAATTTTGTATATAGATTTTTGTTATTTGCTTCACACGTGAAATATAAATTT
TACCTGATGTAACAATTAGGCAGATAAGGAAAGCAAACTTCATTGGTATTTTTTTACTTT
ACAATATATAGCGGAAGTTGTTACACAAAACTCGGGGTACTCCCCAATACGTAGAAGTAC
AACTATTTCTAGATACAATAGTTATATAAAGGGATCTACTGTAAATTACTAATTATTTTG
TTTTTGTTACCAGCCAAATAAATACTTATCAAATTACAAGGAAAAAGTCCAGTTATTGAC
TAAGTAAAATTATATGAATTTATTACAAAATTTTGTAAATATACATGAGACTAGTATAAC
TGATATTCTACAAAAAGTCGGTAACAAACTAACAATATAAGACCAATTCTAGAGTGCAAA
CTAGAAAACAAACAGTTTAAGAGTTACAAGAGATAGATCATATAGTTTTGAAATATGGAA
GCAAATAACAATCATCAATCCATATTCTATATTGTAGTTTTTATTTGAATTG

Last Updated on 06.12.2018 16:11