Sequences from confirmation amplicon


Confirmation sequences for line 591B09 and hit At5g01380 are shown.
Line ID 591B09
Locus At5g01380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:156291
Primers used for PCR 06fj - 8474, show primer details
   
Sequence Name 85-K111271-0022-591-B09C-06fj
GenBank Accession LN514561 [GenBank]
Sequencing primer gene specific primer, 06fj: 5'-AATCTCTCTTCTGAGCCCTAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K111271-0022-591-B09C-06fj
CATCACCTCCTTTCTGTGGCGGCTCTTTCCTCCTCTAGCTCCGCCATTCTCCTCCGCCAC
TCTTTCTCTCTCTTCTCCCTCTCTATCTCCTTCATCTCCCATGCATCTCTCCATTCCTTT
TCCATCTTCACCGTTTGCCTCATAAACTCCTCCAATATATCTTTTAGTGTATTACCCGCA
GTTTCAGCTTTCGTACCACTCGCAACGCCTTTTCCTTTTTTTGCACGTTTTCTTGGATTG
GTCGAAGTACTAGTAGTAATCACTTCTGTCTCTCTTTTCTGTGTCTCTACTAAAGACAAT
AATTCCTCGTTGATATCTTGATTTGGTTCGTCAACTTCTTCTTCTTCATCATCAGATGAA
AATTGATGATGTTTTCTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCACAATTATT
   
Sequence Name 70-K111270-0022-591-B09C-8409
GenBank Accession LN512413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K111270-0022-591-B09C-8409
GCCTTTACATTGAAGAGAAACATCATCAATTTTCATCTGATGATGAAGAAGAAGAAGTTG
ACGAACCAAATCAAGATATCAACGAGGAATTATTGTCTTTAGTAGAGACACAGAAAAGAG
AGACAGAAGTGATTACTACTAGTACTTCGACCAATCCAAGAAAACGTGCAAAAAAAGGAA
AAGGCGTTGCGAGTGGTACGAAAGCTGAAACTGCGGGTAATACACTAAAAGATATATTGG
AGGAGTTTATGAGGCAAACGGTGAAGATGGAAAAGGAATGGAGAGATGCATGGGAGATGA
AGGAGATAGAGAGGGAGAAGAGAGAGAAAGAGTGGCGGAGGAGAATGGCGGAGCTAGAGG
AGGAAAGAGCCGCCACAGAAAGGAGGTGGATGGAGAGAGAGGAAGAGAGACGGTTGAGAG
AAGAAGCTAGGGCTCAGAAGAGAGAT

Last Updated on 06.12.2018 16:11