Sequences from confirmation amplicon


Confirmation sequences for line 591E08 and hit At4g34370 are shown.
Line ID 591E08
Locus At4g34370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16434838
Primers used for PCR NU71 - 8474, show primer details
   
Sequence Name 34-K104096-0022-591-E08C-NU71
GenBank Accession LN501393 [GenBank]
Sequencing primer gene specific primer, NU71: 5'-AAGAAGAAAATATCCATCAATGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104096-0022-591-E08C-NU71
AATTAAAAAAAACTTTCACAATTATGGCAAAAAAACACTCTTCACATTCGGACCAAAACG
ACGACGTAACATAAATGTATCGTTTTGCATAAAAGCAGTAAAAGTGAGAGAGAGAGAGAG
ACAGATAGGAATCTTTGAGCGATTCGTCTTCCTCCTCTATCGCCCTCTACTCTTCACCGG
AACTGTGATCTCACCGGAATATCCCTCCTTTATTTATCCTCTACTCATCCGTTTTACTCT
CTCCCTCCGTCGATCTTCTCTTTCTCTTGATCGCCTCCGTCCATTCTTTATCATTTCTTA
ATTCAGAATTGTGATCGATGGATGATTATTTTAGCGCGGAGGAAGAGGCTTGCTATTACT
CCTCCGATCAAGATTCTTTGGATGGAATTGATAATGAAGAGTCTGAATTGCAGCCTCTGT
CCTCTAAAAGATCAAACACTCAGGTTCTCTTATATACACTCTTCTTCCTCTCTTCATTCT
TCTATCTCTTTCTTCTTAATATCCGTGATTGTTTCATATTTGGTTGTTATTGGTAAGTGG
ATTGATTGTTTCATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGC
   
Sequence Name 50-K104097-0022-591-E08C-8409
GenBank Accession LN507149 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104097-0022-591-E08C-8409
TGAAGCCATTTACAATTGATGAAACAATCAATCCACTTACCAATAACAACCAAATATGAA
ACAATCACGGATATTAAGAAGAAAGAGATAGAAGAATGAAGAGAGGAAGAAGAGTGTATA
TAAGAGAACCTGAGTGTTTGATCTTTTAGAGGACAGAGGCTGCAATTCAGACTCTTCATT
ATCAATTCCATCCAAAGAATCTTGATCGGAGGAGTAATAGCAAGCCTCTTCCTCCGCGCT
AAAATAATCATCCATCGATCACAATTCTGAATTAAGAAATGATAAAGAATGGACGGAGGC
GATCAAGAGAAAGAGAAGATCGACGGAGGGAGAGAGTAAAACGGATGAGTAGAGGATAAA
TAAAGGAGGGATATTCCGGTGAGATCACAGTTCCGGTGAAGAGTAGAGGGCGATAGAGGA
GGAAGACGAATCGCTCAAAGATTCCTATCTGTCTCTCTCTCTCTCTCACTTTTACTGCTT
TTATGCAAAACGATACATTTATGTTACGTCGTCGTTTTGGTCCGAATGTGAAAGTGTTTT
TTTTGCCATAATTGTGAAAGTTTTTTTTAATACTAAGCGCATTGATGGATATTTTCTTC
Primers used for PCR NU72 - 8474, show primer details
   
Sequence Name 34-K104270-0022-591-E08C-NU72
GenBank Accession LN501401 [GenBank]
Sequencing primer gene specific primer, NU72: 5'-TTGATAATCGAAGACAGTGACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104270-0022-591-E08C-NU72
TTTTCTTTCTCACAAACACAGCAACAACTTCTCCCATCCCACTGGTAATGAATAAGAAGA
GTCCGTGCATGGTGCTCCTTTATTGATAACAATTCCATCACCCTTAGCAAATCTTCTCTC
TGCATCAGAAAACCAATAACTCAAACCAAACTAAAGCTTAAAATTAAACCCGAGAGTAAA
ATCATCTCTGCTGATTTTACCTGTGCTGCTAGAAGCGATTCCTGAGTGATGACCTGCAAA
ATTGTAGAATAACTGTTAACAAACCCTAATTTCAAACAGAGAAGAACAGAACAGAACAAT
TCTGCAATAAATTAGAATCCTAAACACTGTGAAAAATGAGAAATTCAGATGAACAATGAT
GCCGGAACCAGATTTTCAATCTCACAATTGCAATGAAATTCACTTTGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 50-K104271-0022-591-E08C-8409
GenBank Accession LN507157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104271-0022-591-E08C-8409
TTTATTGCATTGTGAGATTGAAATCTGGTTCCGGCATCATTGTTCATCTGAATTTCTCAT
TTTTCACAGTGTTTAGGATTCTAATTTATTGCAGAATTGTTCTGTTCTGTTCTTCTCTGT
TTGAAATTAGGGTTTGTTAACAGTTATTCTACAATTTTGCAGGTCATCACTCAGGAATCG
CTTCTAGCAGCACAGGTAAAATCAGCAGAGATGATTTTACTCTCGGGTTTAATTTTAAGC
TTTAGTTTGGTTTGAGTTATTGGTTTTCTGATGCAGAGAGAAGATTTGCTAAGGGTGATG
GAATTGTTATCAATAAAGGAGCACCATGCACGGACTCTTCTTATTCATTACCAGTGGGAT
GTGGAGAAGTTGTTTGCTGTGTTTGTTGAGAAAGGAAAAGATAGCTTGTTTTCTGGAGCT
GGTGTCACTGTCTTCGATTATCA

Last Updated on 06.12.2018 16:11