Sequences from confirmation amplicon


Confirmation sequences for line 591G03 and hit At5g43420 are shown.
Line ID 591G03
Locus At5g43420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17452831
Primers used for PCR CN61 - 8474, show primer details
   
Sequence Name 50-K104163-0022-591-G03C-CN61
GenBank Accession LN507152 [GenBank]
Sequencing primer gene specific primer, CN61: 5'-GAGGATTATCCCAAATTGTTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104163-0022-591-G03C-CN61
CGATGTGTGGCTTCGAACACGCCAATTGTCCTTTGTGTAGAACTAGGGTTTCTTGTGACA
CAAGTTTTCCTCCGGATCGGGTTTCTGCGCCGAGCACTTCTCCCGAGAATCTGGTCATGT
TAAGAGGTGAGAACGAGTATGTGGTCATTGAGCTGGGCAGTAGCATCGGTAGTGACAGAG
ATAGTCCAAGACACGGAAGGTTACTTACGGGACAAGAAAGGTCAAATTCAGGTTATCTAC
TGAACGAAAACACCCAAAATTCGATCAGTCCATCTCCGAAGAAGCTTGACCGCGGAGGGC
TTCCAAGAAAATTCCGAAAGCTTCACAAGATGACGAGTATGGGAGACGAATGCATCGACA
TAAGAAGAGGTAAAGACGAACAGTTCGGTAGTATTCAGCCCATTAGAAGATCAATCTCAA
TGGATTCATCGGCGGATAGACAGCTTTACTTGGCGGTTCAAGAGGCGATTCGGAAAAACC
GCGAAGTTCTGGTGGTTGGAGACGGAGGAGGATGTAGCAGTAGTAGTGGCAATGTTAGTA
ATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCC
   
Sequence Name 50-K104164-0022-591-G03C-8409
GenBank Accession LN507153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104164-0022-591-G03C-8409
GCCATTTACATTGAATATATCCTGCAATTACTAACATTGCCACTACTACTGCTACATCCT
CCTCCGTCTCCACCACCAGAACTTCGCGGTTTTTCCGAATCGCCTCTTGAACCGCCAAGT
AAAGCTGTCTATCCGCCGATGAATCCATTGAGATTGATCTTCTAATGGGCTGAATACTAC
CGAACTGTTCGTCTTTACCTCTTCTTATGTCGATGCATTCGTCTCCCATACTCGTCATCT
TGTGAAGCTTTCGGAATTTTCTTGGAAGCCCTCCGCGGTCAAGCTTCTTCGGAGATGGAC
TGATCGAATTTTGGGTGTTTTCGTTCAGTAGATAACCTGAATTTGACCTTTCTTGTCCCG
TAAGTAACCTTCCGTGTCTTGGACTATCTCTGTCACTACCGATGCTACTGCCCAGCTCAA
TGACCACATACTCGTTCTCACCTCTNAACATGACCAGATTCTCGGGAGAAGTGCTCGGCG
CAGAAACCCGATCCGGAGGAAAACTTGTGTCACAAGAAACCCTAGTTCTACACAAAGGAC
AATTGGCGTTGTTCTGAAGCCACACATCGATACAGTCGATATGAAACAAATGAGAACAAT
T

Last Updated on 06.12.2018 16:11