Sequences from confirmation amplicon


Confirmation sequences for line 592A10 and hit At1g35140 are shown.
Line ID 592A10
Locus At1g35140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12852889
Primers used for PCR HM84 - 8474, show primer details
   
Sequence Name 67-K101271-0022-592-A10C-8409
GenBank Accession LN511767 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K101271-0022-592-A10C-8409
CGGAATGAGCCTTTACATTGAATATATCATGTAGATTTACCAGGATACGAACCTTTCCCA
TAGACTCCAGTACAAGCCGATCCAGCCTCAAGCGGTGCCGTTTTAGGCCCTTGGTAATAT
CCATCTCCGAACGGGTTCGTTGCCGTCGCAGCCATGAGACTCGCCAAGTTAATCACCATC
CCGTCTAAACCAACGTCGTTGTTTGGTGCCACTAGTGGTGGGCTTTGCGGTCCGTAAACC
GGCGCGTGGAATGGCCACGCGCATTGTCCTGGACATTGTGTTTCTGAGTTTCCAACCCAG
ATGTAAGCGAATCTTGATCCTTTCTTGCCTGACCCGGAACCAGTCCCGTGTGACCCGCAT
CTGTTCATGCAAAAGCCTTGGACCGTCACGTCAGCTGAGGTCAACACGACGTTNACCGCG
TAGCTTTGGCCACCTTTTGCGGCCAAGTCTTTGAGGTTTTTCTCTGTTAAAGATTTTCCC
ATTGAGTATCCTTGGTCGAGGATCTGTTCTCCGAGGGAGAGACTGAGTCCACGTGTCGTG
GTCATCTTGCGGAATGGTAATAC
   
Sequence Name 67-K101277-0022-592-A10C-HM84
GenBank Accession LN511768 [GenBank]
Sequencing primer gene specific primer, HM84: 5'-AAGTATTACCAATTCCGCAAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K101277-0022-592-A10C-HM84
CAGATCCTCGACCAGGATACTCAATGGGAAAATCTTTAACAGAGAAAAACCTCAAAGACT
TGGCCGCAAAAGGTGGCCAAAGCTACGCGGTTAACGTCGTGTTGACCTCAGCTGACGTGA
CGGTCCAAGGCTTTTGCATGAACAGATGCGGGTCACACGGGACTGGTTCCGGGTCAGGCA
AGAAAGGATCAAGATTCGCTTACATCTGGGTTGGAAACTCAGAAACACAATGTCCAGGAC
AATGCGCGTGGCCATTCCACGCGCCGGTTTACGGACCGCAAAGCCCACCACTAGTGGCAC
CAAACAACGACGTTGGTTTAGACGGGATGGTGATTAACTTGGCGAGTCTCATGGCTGCGA
CGGCNACGAACCCGTTCGGAGATGGATATTACCAAGGGCCTAAAACGGCACCGCTTGAGG
CTGGATCGGCTTGTACTGGAGTCTATGGGAAAGGTTCGTATCCTGGTAAATCTACATGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11