Sequences from confirmation amplicon


Confirmation sequences for line 592F04 and hit At5g17760 are shown.
Line ID 592F04
Locus At5g17760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5860955
Primers used for PCR 39xc - 8474, show primer details
   
Sequence Name 44-K119070-0022-592-F04C-39xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 39xc: 5'-ACACCATTGTTTTCGACTTTTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K119070-0022-592-F04C-39xc
AGATTTGTATTGGGAGATAATTCTCTCTCTTTATTCATCTTTTTCATCATTTGGGCCACA
AAGGCCAACCCATTAGGCTAATAACGCAAAGTACGTATCAAGCTATTACGCAGTTTGGGA
TATTCATTACCACATAAATAAAAGAAGATATCAACACTTAAAGATTTTACTCTTCTCAAA
AGTCTCTGTAACAAGGCAAGAGACTCTCCACTCTATATGCTTTATTTTTTCTTTGGTTCT
TCCTTTATATAGGTAGAGTATAACATACTTGGTAGTTGGTAATGTACCTCCAAAGACTAT
CTCAATATATAGTTTCTTACTTTAAGGAAAAATAAAGTTCCATATCTCTTAATGGGTCTT
ACAATGGTTCACAACCCTAGAAAATAAACATACAAGAAATTTCTTTTAAAAAACACTTAA
AGAGTTGTAATTAGCCTCTTATACGTCTATTTTTGTTTGCTTCCTCATAATCTTATTTGA
ACTAATTAATGGATTATGTCCAAAACAATACAAGCTTCTTAAATCTTTTGCTGTTCTTCC
TCGGAGAACCCTCAGTATCACTCTTTAGTCTCATCTCCTCCATCTCCAGTCTACTCTCTT
TCTGCTTCATCATCACCGGATTCGATTCCTTAGATTTTAGCCTCATTTTCTCTAAAACAT
TCACCAAACCCTCTAGCGCCACGTCAGCATCCTCACTCTTCATCAGCTCCTCTGCTACTT
GTGCCGGCGTCATTACTTCCCCGTCAATCAAACGCTCAATCTCCGGAAATAGACGGTGTG
GCAT
   
Sequence Name 12-K119069-0022-592-F04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K119069-0022-592-F04C-8409
TTTACATGGGACATTGCTCTTTTCAAGGATTCAGACTTTAGCTTCTAACTACTTGGGTTT
GAGTGATGCTGCGATGCCACACCGTCTATTTCCGGAGATTGAGCGTTTGATTGACGGGGA
AGTAATGACGCCGGCACAAGTAGCAGAGGAGCTGATGAAGAGTGAGGATGCTGACGTGGC
GCTAGAGGGTTTGGTGAATGTTTTAGAGAAAATGAGGCTAAAATCTAAGGAATCGAATCC
GGTGATGATGAAGCAGAAAGAGAGTAGACTGGAGATGGAGGAGATGAGACTAAAGAGTGA
TACTGAGGGTTCTCCGAGGAAGAACAGCAAAAGATTTAAGAAGCTTGTATTGTTTTGGAC
ATAATCCATTAATTAGTTCAAATAAGATTATGAGGAAGCAAACAAAAATAGACGTATAAG
AGGCTAATTACAACTCTTTAAGTGTTTTTTAAAAGAAATTTCTTGTATGTTTATTTTCTA
GGGTTGTGAACCATTGTAAGACCCATTAAGAGATATGGAACTTTATTTTTCCTTAAAGTA
AGAAACTATATATTGAGATAGTCTTTGGAGGTACATTACCAACTACCAAGTATGTTATAC
TCTACCTATATAAAGGAAGAACCAAAGAAAAAATAAAGCATATAGAGTGGAGAGTCTCTT
GCCTTGTTACAGAGACTTTTGAGAAGAGTAAAATCTTTAAGTGTTGATATCTTCTTTTAT
TTATGTGGTAATGAATATCCCAAACTGCGTAATAGCTTGATACGTACTTTGCGTTATTAG
CCTAATGGGCTGCCCTTTGTGGCCCAAATGATGAAAAAGATGAATAAAGAGAGAGAATTA
TCTCCCAATACAAATCTCTATATAATAAACCCCATTTACCAAAAGAGAAACCAAACCCTA
AAGT

Last Updated on 06.12.2018 16:11