Sequences from confirmation amplicon


Confirmation sequences for line 596A04 and hit At1g62085 are shown.
Line ID 596A04
Locus At1g62085 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22949662
Primers used for PCR 43tv - 8474, show primer details
   
Sequence Name 56-K117042-0022-596-A04C-43tv
GenBank Accession LN509217 [GenBank]
Sequencing primer gene specific primer, 43tv: 5'-CATCTCCAACAGAAAAGCCTAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117042-0022-596-A04C-43tv
TACTTTTGCTTCAAGTTTTGTCCGGATCTGTAACAACCCTAAGAGCTTTGACAAACTTTG
AGGTGGTTGGATCAAAACCCATCTCAACAACTTTCTTGAGTGATTCTTCAAAATTTTCTT
TTCCACAGACAGGTTGGTGATCAGATATAAGCAATGGGAACAACAACCTTTGAGGAACAC
CCAATTCTCTCAAAACCAAAACATTTCTGATTTTGTTCTCCTGCTTACTACCCTCTGGCA
AAGAATGACATAACTTTTCGAACTTGGAACTCTTATCCGCTTCTATAATCTCTTTAACAA
AATCATAGTATATTCAATATGGAGAAAAAGTCCGGGAAATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGATTATTAT
   
Sequence Name 26-K117041-0022-596-A04C-8409
GenBank Accession LN498099 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117041-0022-596-A04C-8409
TTGAATATTTCCCGGACTTTTTCTCCATATTGAATATACTATGATTTTGTTAAAGAGATT
ATAGAAGCGGATAAGAGTTCCAAGTTCGAAAAGTTATGTCATTCTTTGCCAGAGGGTAGT
AAGCAGGAGAACAAAATCAGAAATGTTTTGGTTTTGAGAGAATTGGGTGTTCCTCAAAGG
TTGTTGTTCCCATTGCTTATATCTGATCACCAACCTGTCTGTGGAAAAGAAAATTTTGAA
GAATCACTCAAGAAAGTTGTTGAGATGGGTTTTGATCCAACCACCTCAAAGTTTGTCAAA
GCTCTTAGGGTTGTTTACAGATTCCGCGACAAAACTATAGAAGCAAAAGTTAATGTCTGT
AAAAGTTTAGGCTTTTCTGTTGGAGATG

Last Updated on 06.12.2018 16:11