Sequences from confirmation amplicon


Confirmation sequences for line 596B05 and hit At1g53870 are shown.
Line ID 596B05
Locus At1g53870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20112592
Primers used for PCR 62xe - 8474, show primer details
   
Sequence Name 45-K119070-0022-596-B05C-62xe
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 62xe: 5'-TGTATTTGCCTGATTCGTTGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K119070-0022-596-B05C-62xe
TTCACATTCGTCCACTCTTTTACCCGTCTCGTACCTTTCTCACATCCGCTAGGAGATTTT
TATATATCCTGAAAAATTCAAGTAATTTTCTTAATTTCCAAAGTATCTCCAAATAGGCCA
AAATGTGAAAGCAACGACTCAACATTGTCTTCTAGGTTGCAACAAGTATAGTTTAATTAA
TTGATGATATAGCGGGACGTTGGAAATCCTTCAAGCAGCCAAAATATGTGACCAATTGAA
GAGGACGTGATGAATTGTCTCCCTAACTTGGCACCATCGTTAGCAACATGGGGTCAATGG
TTATTCCTATAGAACTTCTTTTTGTGCACTATCATATCAAGAAACTGTCAGCATTTACCA
GCAACGAGAATGCAAATGCAATGAACCGAAATGGACGACCATTTCTAAGCTTATCAACTA
ATAGTTCTATTACTATATACTATATTGTATGTGACGATGTGCACGATATATATTTTTCTA
ATACTCGAATCTCACACGTGACAACGACCTTACGTGTATGTACGTGTTGAATAGAATTAA
GATACGTTTTATAAACTTCTCTTATCTCACATATTCAACATGTGGATGAAGATCTTCGTA
TTTTTTATTTTAATTTTTATGATACACCCATAAACTGAATTATTACTAGCTAGTAGAGGC
TATAAATACGAAGAAGATATCTATATATATACTATCTATCAGTGCCAAAAAAAAGTTTTT
CAATATATGCAAATATACAATCCTATTTCTCCGGTGAAAACCATGGTGAAGATTTATCCT
CACCTGGCGTTTCCGGTGGATATGGAC
   
Sequence Name 13-K119069-0022-596-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119069-0022-596-B05C-8409
CATTTCAATTGAATATACATGAAGCCTTTTGACGTAAAGTAAACAAAATTTTGCCGTTTA
AATCCATAAAGTAAACTTCATTACTCCAACTCTTGGAATCATAATTATCCACTCGATAGA
TTAAGTTTCCTTTGGAATCAAAAACTGTGCAGCCTTTGCTATTGAACACCAAAGATTTCA
TCCAAATCGTGAACCTCTCTTGCTCCGTCGTTAAGTAGGGCAAAATCTTGTCTTGTACGA
CGTCCATATCCACCGGAAACGCCAGGTGAGGATAAATCTTCACCATGGTTTTCACCGGAG
AAATAGGATTGTATATTTGCATATATTGAAAAACTTTTTTTTGGCACTGATAGATAGTAT
ATATATAGATATCTTCTTCGTATTTATAGCCTCTACTAGCTAGTAATAATTCAGTTTATG
GGTGTATCATAAAAATTAAAATAAAAAATACGAAGATCTTCATCCACATGTTGAATATGT
GAGATAAGAGAAGTTTATAAAACGTATCTTAATTCTATTCAACACGTACATACACGTAAG
GTCGTTGTCACGTGTGAGATTCGAGTATTAGAAAAATATATATCGTGCACATCGTCACAT
ACAATATAGTATATAGTAATAGAACTATTAGTTGATAAGCTTAGAAATGGTCGTCCATTT
CGGTTCATTGCATTTGCATTCTCGTTGCTGGTAAATGCTGACAGTTTCTTGATATGATAG
TGCACAAAAAGAAGTTCTATAGGAATAACCATTGACCCCATGTTGCTAACGATGGTGCCA
AGTTAGGGAGACAATTCATCACGTCCTCTTCAATTGGTCACATATTTTGGCTGCTTGAGG
GATTTTCAACGTCCCGCTATATCATCCATTAATTAAACTATACTTGTTGCA
Primers used for PCR 75xc - 8474, show primer details
   
Sequence Name 46-K119070-0022-596-B05C-75xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 75xc: 5'-CAAGAAACTGTCAGCATTTACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119070-0022-596-B05C-75xc
ACCATTTCTAGCTTATCGACTAATAGTTCTATTACTATATACTATATTGTATGTGACGAT
GTGCACGATATATATTTTTCTAATACTCGAATCTCACACGAGACAACGACCTTACGTGTA
TGTACGTGTTGAATAGAATTAAGATACGTTTTATAAACTTCTCTTATCTCACATATTCAA
CATGTGGATGAAGATCTTCGTATTTTTTATTTTAATTTTTATGATACACCCATAAACTGA
ATTATTACTAGCTAGTAGAGGCTATAAATACGAAGAAGATATCTATATATATACTATCTA
TCAGTGCCAAAAAAAAGTTTTTCAATATATGCAAATATACAATCCTATTTCTCCGGTGAA
AACCATGGTGAAGATTTATCCTCACCTGGCGTTTCCGGTGGATATGGACGTCGTACAAGA
CAAGATTTTGCCCTACTTAACGACGGAGCAAGAGAGGTTCACGATTTGGATGAAATCTTT
GGTGTTCAATAGCAAAGGCTGCACAGTTTTTGATTCCAAAGGAAACTTAATCTATCGAGT
GGATAATTATGATTCCAAGAGTTGGAGTAATGAAGTTTACTTTATGGATTTAAACGGCAA
AATTTTGTTTACTTTACGTCAAAAGGCTTCATGTATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K119069-0022-596-B05C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K119069-0022-596-B05C-oriL
CATTACATTGAATATACATGAAGCCTTTTGACGTAAAGTAAACAAAATTTTGCCGTTTAA
ATCCATAAAGTAAACTTCATTACTCCAACTCTTGGAATCATAATTATCCACTCGATAGAT
TAAGTTTCCTTTGGAATCAAAAACTGTGCAGCCTTTGCTATTGAACACCAAAGATTTCAT
CCAAATCGTGAACCTCTCTTGCTCCGTCGTTAAGTAGGGCAAAATCTTGTCTTGTACGAC
GTCCATATCCACCGGAAACGCCAGGTGAGGATAAATCTTCACCATGGTTTTCACCGGAGA
AATAGGATTGTATATTTGCATATATTGAAAAACTTTTTTTTGGCACTGATAGATAGTATA
TATATAGATATCTTCTTCGTATTTATAGCCTCTACTAGCTAGTAATAATTCAGTTTATGG
GTGTATCATAAAAATTAAAATAAAAAATACGAAGATCTTCATCCACATGTTGAATATGTG
AGATAAGAGAAGTTTATAAAACGTATCTTAATTCTATTCAACACGTACATACACGTAAGG
TCGTTGTCACGTGTGAGATTCGAGTATTAGAAAAATATATATCGTGCACATCGTCACATA
CAATATAGTATATAGTAATAGAACTATTAGTTGATAAGCTTAGAAATGGTCGTCCATTTC
GGTTCATTGCATTTGCATTCTCGTTGCTGGTAAA

Last Updated on 06.12.2018 16:11