Sequences from confirmation amplicon


Confirmation sequences for line 596D07 and hit At2g24950 are shown.
Line ID 596D07
Locus At2g24950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10613494
Primers used for PCR NE72 - 8474, show primer details
   
Sequence Name 76-K104224-0022-596-D07C-NE72
GenBank Accession LN513393 [GenBank]
Sequencing primer gene specific primer, NE72: 5'-CGAATATGAAAATGAGTTGTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K104224-0022-596-D07C-NE72
GTAATCCTGTATTTTTATCGTTTTGCGGATGCTGAAAAATACAAACTTCCAGTGATTTTG
AAATTGAAGAGCAACTTAGAGTCGTCAACAAACCTGCTACGAAAATTATCAAGGTCCCTT
TACTTTTTATCTTAAACTCTAGAGCCATATTATTTACGTCGAATTGTACATATAAATTAA
TACATGTTCTTATACAATGATATAAAAAGTTTACAAAATTATTTCTAATCTAATTTGTTT
TTGGATGATAGACTATACATGGCGATTCATATGATTGCGTACATTTTAAATCCACATGGA
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAANCTCGCTGAACCATGATTTAGATTTTCAGGATTCAGTCCCAGG
TGAAGTACTTGCCGGTTTGTTCTC
   
Sequence Name 36-K104225-0022-596-D07C-8409
GenBank Accession LN502210 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104225-0022-596-D07C-8409
GATTCATGTGGATTTAAAATCTACGCGATCATATGAATCGCCATGTATAATCTATCATCC
AAAAACAAATTAGATTACAAATAATTTTGTATACTTTTTATATCATTGTATAAGAACATG
AATTAATTTATATGTACAATTCGACGTAAATAATATGGCTCTAGAGTTTAAGATAAAAAG
TAAAGGGACCTTGATAATTTTCGTAGCATGTTTGTTGACGACTTTAAGTTGCTCTTCAAT
TTCAAAATCACTGAAAGTTTGTAATATTTCAGCATCCACAAAACGATAAAAATACAAGAT
ATAACCCAACAAGAATAGCAACATCCTCCAACAACTCATTTTCATATTC

Last Updated on 06.12.2018 16:11