Sequences from confirmation amplicon


Confirmation sequences for line 596F04 and hit At5g14310 are shown.
Line ID 596F04
Locus At5g14310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4615871
Primers used for PCR 49hw - 8474, show primer details
   
Sequence Name 51-K112689-0022-596-F04C-49hw
GenBank Accession LN507553 [GenBank]
Sequencing primer gene specific primer, 49hw: 5'-TGATCAATTTTGACTCTTTGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112689-0022-596-F04C-49hw
GATCGGGTCATTTCAAGTTGGGAAACAAACCCTAGCGAACCTCCATTGATGTGATCTCTC
TCTCTCTCTCACAACTTGAGAATCAACTCTCTAGAAAATATCAGAAAATCGTTGAAAAAA
TCTGGTCTTTTCTGGAGGGAAATGCCAGGGGTAGCTGTGAAGCTCTATAGCGTCTTCTTT
AAGTTACTATTGAAACATCGTCTTCAGAATCTGATTTCAATTTCAGCTGCTGATGGCTTA
TCTGATTCCTTTGGTGTTTCGACCCGATCCGATGAATCCGTCGCCGCCGCGAATCCGTCT
TTCACCGACGGTGTTGCCACCAAAGATATACACATTGATCCAATGACGTCACTCACCGTA
AGGATCTTTCTTCCCGAATCTGCTCTTTCTCCAGAACCAGATTCGTTACGCCACAAGGAT
ATCCGGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
C
   
Sequence Name 19-K112688-0022-596-F04C-8409
GenBank Accession LN494830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112688-0022-596-F04C-8409
CATTTCCGGATATCCTTGTGGCGTAACGAATCTGGTTCTGGAGAAAGAGCAGATTCGGGA
AGAAAGATCCTTACGGTGAGTGACGTCATTGGATCAATGTGTATATCTTTGGTGGCAACA
CCGTCGGTGAAAGACGGATTCGCGGCGGCGACGGATTCATCGGATCGGGTCGAAACACCA
AAGGAATCAGATAAGCCATCAGCAGCTGAAATTGAAATCAGATTCTGAAGACGATGTTTC
AATAGTAACTTAAAGAAGACGCTATAGAGCTTCACAGCTACCCCTGGCATTTCCCTCCAG
AAAAGACCAGATTTTTTCAACGATTTTCTGATATTTTCTAGAGAGTTGATTCTCAAGTTG
TGAGAGAGAGAGAGAGATCACATCAATGGAGGTTCGCTAGGGTTTGTTTCCCAACTTGAA
ATTGACCCGATTCGGAGAACAATTACGGAGATTAGAATCGAAATTGGATGCAAAGAGTCA

Last Updated on 06.12.2018 16:11