Sequences from confirmation amplicon


Confirmation sequences for line 597E02 and hit At4g01630 are shown.
Line ID 597E02
Locus At4g01630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:700997
Primers used for PCR 19qt - 8474, show primer details
   
Sequence Name 37-K115781-0022-597-E02C-19qt
GenBank Accession LN502736 [GenBank]
Sequencing primer gene specific primer, 19qt: 5'-CTCCGTATTTTTCGCACTTCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115781-0022-597-E02C-19qt
CAATCATATATATTTTCACGTTTTCCGCTAATTCCAAGTCATATCTAACTAATTTATGCA
TATATCTTAATCCAAACAAGCTCTTTGAGTTCTACTATAAATAGCTGCTAAAGCCTAAGT
CTTATGCATCCAACAATCTTCAACTTCTTCTCTAATCAAAAGTTAAAACACCTAAGAAAT
TGAAAGAAAACAAAATGACGAAGATTTTCTCTCTATTGGTCGCAATGATCTTCTCCACAA
TGTTCTTCATGAAGATCAGCTCAGTTTCTGCTGGTTGGTTGCAAGCTCATGCGACCTTTT
ATGGCGGAAGTGATGCCTCTGGTACAATGGGTAAGTCTCATAAACTAGACATGAACATTT
GGTTTTACCCTAATGAGAGCTTTAAAAAATAATGTTCCTTCTTCAACAAGAAACCTTCTC
ATAGCTTTCTTCTTCAGGTGGAGCTTGTGGTTATGGAAACCTATACACAGACGGCTACNA
GACAAACACCGCGGCGTTAAGCACGGCCCTGTTCAACGACGGCNAGTCATGCGGTGGATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
T
   
Sequence Name 13-K115780-0022-597-E02C-8409
GenBank Accession LN492186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115780-0022-597-E02C-8409
TATCCACCGCATGACTTGCCGTCGTTGAAAGGGCCGTGCTTAACGCCGCGGTGTTTGTCT
TGTAGCCGTCTGTGTATAGGTTTCCATAACCACAAGCTCCACCTGAAGAAGAAAGCTATG
AGAAGGTTTCTTGTTGAAGAAGGAACATTATTTTTTAAAGCTCTCATTAGGGTAAAACCA
AATGTTCATGTCTAGTTTATGAGACTTACCCATTGTACCAGAGGCATCACTTCCGCCATA
AAAGGTCGCATGAGCTTGCAACCAACCAGCAGAAACTGAGCTGATCTTCATGAAGAACAT
TGTGGAGAAGATCATTGCGACCAATAGAGAGAAAATCTTCGTCATTTTGTTTTCTTTCAA
TTTCTTAGGTGTTTTAACTTTTGATTAGAGAAGAAGTTGAAGATTGTTGGATGCATAAGA
CTTAGGCTTTAGCAGCTATTTATAGTAGAACTCAAAGAGCTTGTTTGGATTAAGATATAT
GCATAAATTAGTTAGATATGACTTGGAATTAGCGGAAAACGTTGAAAATAATAATATGAT
TTGGTGATGATTGATTCAATGATTGTAAGAAGTGCGAA

Last Updated on 06.12.2018 16:11