Sequences from confirmation amplicon


Confirmation sequences for line 597G07 and hit At5g26120 are shown.
Line ID 597G07
Locus At5g26120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9124185
Primers used for PCR FV34 - 8474, show primer details
   
Sequence Name 55-K033884-022-597-G07C-8409
GenBank Accession LN508727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K033884-022-597-G07C-8409
GAAGCCATTTACAATTGATTCAATGAAGTAAACAAACCTTGTAAGTATCCATAGGCATGG
CTGAGACTTGATCAAACCAAATTGAACCTTTTTTGGTAGTTGTAAACTGAAGTCTTGCAC
CATTATCTGTTCCATTTGCTTCTAAAAGCATTTCCTTTTTTGTCCAATTTAAAAGATCAG
AAGCCAAAGCTCTACAGAAATAAATGAAAAACATCATTGAAAAAACATTCAGAAAAATAT
TGGAGGATGTGAAATTTCATTTCTCACATAATATTTTCTGACGCAAGAGTCACTGATCCA
TTAGAACTTGTAAACGACACAGACACATCGATGTCCCCAGTCGAACGCACATATAACACC
ACTTTATATTTCTTCCCTTCTTCAATGTTCTAATAGAAAAGATTTACAAATGAGTAAAAA
CTTTTAAGCATTAGAGGAAATGAAAATTGTGATTGAGAGTACAAAAATGTTACCATGCCC
CAGTAACCCGGGTTATAAACCCCGACTCCTCCTAACGGACAAGAGTTGCTGTCACAAAGG
ACTTCCATTCTAAGAGCAATCTTGTTACGCTCAAAACACGAAGAACGGTCTGAAACAACA
T
   
Sequence Name 15-K033884-022-597-G07C-FV34
GenBank Accession LN492875 [GenBank]
Sequencing primer gene specific primer, FV34: 5'-TATGTTGTTACAGACCGTTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K033884-022-597-G07C-FV34
GCTCTTAGAATGGAAGTCCTTTGTGACAGCAACTCTTGTCCGTTAGGAGGAGTCGGGGTT
TATAACCCGGGTTACTGGGGCATGGTAACATTTTTGTACTCTCAATCACAATTTTCATTT
CCTCTAATGCTTAAAAGTTTTTACTCATTTGTAAATCTTTTCTATTAGAACATTGAAGAA
GGGAAGAAATATAAAGTGGTGTTATATGTGCGTTCGACTGGGGACATCGATGTGTCTGTG
TCGTTTACAAGTTCTAATGGATCAGTGACTCTTGCGTCAGAAAATATTATGTGAGAAATG
AAATTTCACATCCTCCAATATTTTTCTGAATGTTTTTTCAATGATGTTTTTCATTTATTT
CTGTAGAGCTTTGGCTTCTGATCTTTTAAATTGGACAAAAAAGGAAATGCTTTTAGAAGC
AAATGGAACAGATAATGGTGCAAGACTTCAGTTTACAACTACCAAAAAAGGTTCAATTTG
GTTTGATCAAGTCTCAGCCATGCCTATGGATACTTACAAGGTTTGTTTACTTCATTGAAT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATT

Last Updated on 06.12.2018 16:11