Sequences from confirmation amplicon


Confirmation sequences for line 598G07 and hit At3g21670 are shown.
Line ID 598G07
Locus At3g21670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7627741
Primers used for PCR CH59 - 8474, show primer details
   
Sequence Name 16-K106998-0022-598-G07C-8409
GenBank Accession LN493451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106998-0022-598-G07C-8409
GAGCCTTTACATTGCTGTTACACAGTCGAAGAAGTGAAACTCGTGATGAAATTGGTACCG
ATTTGGGCAACGAACATTCTTTTCTGGACGATTTACTCCCAAATGACGACTTTCACGGTC
GAACAAGCGACGTTTATGGACCGAAAACTCGGATCTTTCACTGTTCCTGCAGGCTCTTAC
TCTGCTTTCCTCATACTCACAATTCTCCTCTTCACTTCCCTTAACGAGAGAGTCTTTGTT
CCTTTAACAAGAAGGCTCACAAAAAAGCCTCAAGGAATCACAAGCCTACAGAGAATCGGA
GTAGGGCTAGTATTCTCAATGGCTGCAATGGCTGTAGCCGCGGTTATAGAGAACGCTAGA
CGCGAGGCAGCGGTTAACAACGATAAGAAAATAAGCGCGTTTTGGTTGGTTCCACAATAT
TTCTTAGTCGGTGCGGGTGAGGCCTTTGCTTACGTTGGACAGCTTGAGTTCTTTATAAGA
GAAGCACCAGAGAGGATGAAATCGATGAGCACCGGATTGTTTCTAAGCACGATATCGATG
   
Sequence Name 32-K106999-0022-598-G07C-CH59
GenBank Accession LN500604 [GenBank]
Sequencing primer gene specific primer, CH59: 5'-CATCGATATCGTGCTTAGAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106999-0022-598-G07C-CH59
CTTCTCTTATAAAGAACTCAAGCTGTCCAACGTAAGCAAAGGCCTCACCCGCACCGACTA
AGAAATATTGTGGAACCAACCAAAACGCGCTTATTTTCTTATCGTTGTTAACCGCTGCCT
CGCGTCTAGCGTTCTCTATAACCGCGGCTACAGCCATTGCAGCCATTGAGAATACTAGCC
CTACTCCGATTCTCTGTAGGCTTGTGATTCCTTGAGGCTTTTTTGTGAGCCTTCTTGTTA
AAGGAACAAAGACTCTCTCGTTAAGGGAAGTGAAGAGGAGAATTGTGAGTATGAGGAAAG
CAGAGTAAGAGCCTGCAGGAACAGTGAAAGATCCGAGTTTTCGGTCCATAAACGTCGCTT
GTTCGACCGTGAAAGTCGTCATTTGGGAGTAAATCGTCCAGAAAAGAATGTTCGTTGCCC
AAATCGGTACCAATTTCATCACGAGTTTCACTTCTTCGACTTGTGTAACAGCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATT

Last Updated on 06.12.2018 16:11