Sequences from confirmation amplicon


Confirmation sequences for line 599B06 and hit At1g19100 are shown.
Line ID 599B06
Locus At1g19100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6596214
Primers used for PCR 26lx - 8474, show primer details
   
Sequence Name 71-K114129-0022-599-B06C-26lx
GenBank Accession LN512598 [GenBank]
Sequencing primer gene specific primer, 26lx: 5'-GGAAAGCTGGAAGCTATAATGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114129-0022-599-B06C-26lx
CTATATTGCTACTTATCTATATCACTTACTTTTATCTTACTCTTTGCTTGCATGAGTTTG
CTTTTCTCTTTGGAGGTTATACTGTTTTGAGTTATATCCGCCAGAATACATCGTCTCCTT
TGCCTTTTTTTTAATGGTTCTTGACTTATGTTTTGCAGATGGAAAAAATTATCTTCATGT
GCACCCTATGTTCCTTCACTCCAATGCTACCTCACATAAATGGGCTTTTGGAGGTATGCT
TAAACAATTTTCCTTCTTTTGTATGTGTTATTCAAGCGTTTTAACAAGACTTATTTGCTT
CTCTCTATCTGCAGCTGTTGCAGAATTGCTTGACCTAAGAGAAAATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCACGGTT
ATA
   
Sequence Name 29-K114128-0022-599-B06C-8409
GenBank Accession LN499385 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114128-0022-599-B06C-8409
TTGAATATATTTTCTCTTAGGTCAGCAATTCTGCAACAGCTGCTGATAGAGAGAAGCAAA
TAAGTCTTGTTAAAACGCTTGAATAACACATACAAAAGAAGGAAAATTGTTTAAGCATAC
CTCCAAAAGCCCATTTATGTGAGGTAGCATTGGAGTGAAGGAACATAGGGTGCACATGAA
GATAATTTTTTCCATCTGCAAAACATAAGTCAAGAACCATTAAAAAAAAGGCAAAGGAGA
CGATGTATTCTGGCGGATATAACTCAAAACAGTATAACCTCCAAAGAGAAAAGCAAACTC
ATGCAAGCAAAGAGTAAGATAAAAGTAAGTGATAATAGATAAGTAGCAATATAGCATACT
TGGCTGCTGAGACTTTGAACTCAATTCATCATTATAGCTTCCAGCTTTC

Last Updated on 06.12.2018 16:11