Sequences from confirmation amplicon


Confirmation sequences for line 600B02 and hit At1g19450 are shown.
Line ID 600B02
Locus At1g19450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6732124
Primers used for PCR TH30 - 8474, show primer details
   
Sequence Name 42-K103737-0022-600-B02C-TH30
GenBank Accession LN504437 [GenBank]
Sequencing primer gene specific primer, TH30: 5'-AACCGTTGTTCATTCTATACCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103737-0022-600-B02C-TH30
TTGGTGGATAAGCAGGTCGTCGGCTTCTACTCATGGTGAAGAATATTTGAGATTCATCTG
TATATCTTACAAAGTCTGAAGAATTTATGTATGCATAACATAATATTAGCATTTTCTTCT
GAAATGCAGATCTCTTCTATCGGAATGACGATAAGCCTCGTGATTGTAGCAGTTGCATTT
TACCTTAAGGTAACATTTTAACTTTTGACATCTCTACTTGTACAATGTAAGAGTGTCACT
CTAATTATGAGATATTGAGGTTTCTTAATCTTTTGTTAGGAATTTGTATCACCTGATTCC
AACATGTACAACATTCTAAGCATGGTTTCCGTAGTTGGAGTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT
   
Sequence Name 58-K103738-0022-600-B02C-8409
GenBank Accession LN509684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K103738-0022-600-B02C-8409
AAACCATGCTTAGAATGTTGTACATGTTGGAATCAGGTGATACAAATTCCTAACAAAAGA
TTAAGAAACCTCAATATCTCATAATTAGAGTGACACTCTTACATTGTACAAGTAGAGATG
TCAAAAGTTAAAATGTTACCTTAAGGTAAAATGCAACTGCTACAATCACGAGGCTTATCG
TCATTCCGATAGAAGAGATCTGCATTTCAGAAGAAAATGCTAATATTATGTTATGCATAC
ATAAATTCTTCAGACTTTGTAAGATATACAGATGAATCTCAAATATTCTTCACCATGAGT
AGAAGCCGACGACCTGCTTTATCCACCAACCATGTCGCTATCCCAGTCGCCACTACCTGG
TATAGAATGAACCAACGG
Primers used for PCR TH29 - 8474, show primer details
   
Sequence Name 36-K104383-0022-600-B02C-TH29
GenBank Accession LN502214 [GenBank]
Sequencing primer gene specific primer, TH29: 5'-AATGGAGAAAGCAAAAAGTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104383-0022-600-B02C-TH29
TCTGAACAAGCTTGGATCTCTTCAAGCGTTTTTCCTTTAGTCTCAGGAACCCAAAGACTC
ACAAAAACCACAGTGAATCCACAAACCAAAGCGTAGAGAGTGAAAGTTCCCCCACTGCTC
CATGCTAACAGCATATTTGCAGTCATTGTCACTAACCATGACACGAACCAGTTTAACAAA
GTTGCTATACTTCCGGCTAAACCCTTTATATTCACTGGCAGAATCTGTTTTACCAACATT
AATCAGTATTAAAATTTCCAGTTGTGATTTTAACACTAAGGAACACCATGTTTTAATATT
TAGTACCTCAGACATAATCAGCCATGGAATTGGTCCCATTCCTAGAGAGCAAGAAATAAC
CATAGCCTGTAAATAACTCAGCCAGATTTAGTACACATACCGGGAAATATGTAAATAAGA
AAGTATCTTTCGTTGTGTGAATGTATAAAAAGAAAAAGAAAGAAAGAGTAATTACCAATT
AGATGGACATGATTTCCGTTGATCAATTGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTCAATTGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 44-K104384-0022-600-B02C-8409
GenBank Accession LN505174 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104384-0022-600-B02C-8409
CCATTTACATTGAATATATCCTGGATCAATTGAAAAAAATTGGTAATTACTCTTTCTTTT
TCTCCATATTGACCATCATACAATTGATCAACGGAAATCATGTCCATCTAATTGGTAATT
ACTCTTTCTTTCTTTTTCTTTTTATACATTCACACAACGAAAGATACTTTCTTATTTACA
TATTTCCCGGTATGTGTACTAAATCTGGCTGAGTTATTTACAGGCTATGGTTATTTCTTG
CTCTCTAGGAATGGGACCAATTCCATGGCTGATTATGTCTGAGGTACTAAATATTAAAAC
ATGGTGTTCCTTAGTGTTAAAATCACAACTGGAAATTTTAATACTGATTAATGTTGGTAA
AACAGATTCTGCCAGTGAATATAAAGGGTTTAGCCGGAAGTATAGCAACTTTGTTAAACT
GGTTCGTGTCATGGTTAGTGACAATGACTGCAAATATGCTGTTAGCATGGAGCAGTGGGG
GAACTTTCACTCTCTACGCTTTGGTTTGTGGATTCACTGTGGTTTTTGTGAGTCTTTGGG
TTCCTGAGACTAAAGGAAAAACGCTTGAAGAGATCCAAGCTTTGTTCAGATGATCAAAAC
AAGAAGAGGAAGAAGAAGACAAAATTTTCACTTTTTGC

Last Updated on 06.12.2018 16:11