Sequences from confirmation amplicon


Confirmation sequences for line 600C07 and hit At3g58020 are shown.
Line ID 600C07
Locus At3g58020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21480621
Primers used for PCR 19qw - 8474, show primer details
   
Sequence Name 77-K116093-0022-600-C07C-19qw
GenBank Accession LN513600 [GenBank]
Sequencing primer gene specific primer, 19qw: 5'-AAAGATCTTGCAATCTCTCTGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K116093-0022-600-C07C-19qw
GATTGATATGCCACAGTGCAGAGCTTGAATTTTTCCTCAGCTTCATTCTGTGTTTCGTCA
TACCAATTTGTAGTTAGTGTCAAGAAACACGTACAGGGATGCATTCAGTTGCGAAAAACA
CAATAAAGTGGTAAATATAGAGGAGGAAGCTTGTATTAGTTACCTTGGTAGAGGTGTGGT
GACGATCTGGATGCCATTTTAATGCACAAGCTCGGTATCTGAGTAACAAAGTTGGCAAGC
AAGAGTTATGGAGATTAATAAAACATGATCTATGTAAATATAGCTTCTCTCTGATGAGAT
GAAGTGCCTAGTTTTACTTACGCAATTTTGACAATCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 72-K116092-0022-600-C07C-8409
GenBank Accession LN512789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K116092-0022-600-C07C-8409
GCATTTCATTGAATATATCCTGATTGTCAAATTGCGTAAGTAAAACTAGGCACTTCATCT
CATCAGAGAGAAGCTATATTTACATAGATCATGTTTTATTAATCTCCATAACTCTTGCTT
GCCAACTTTGTTACTCAGATACCGAGCTTGTGCATTAAAATGGCATCCAGATCGTCACCA
CACCTCTACCAAGGTAACTAATACAAGCTTCCTCCTCTATATTTACCACTTTATTGTGTT
TTTCGCAACTGAATGCATCCCTGTACGTGTTTCTTGACACTAACTACAAATTGGTATGAC
GAAACACAGAATGAAGCTGAGGAAAAATTCAAGCTCTGCACTGTGGCATATCAATCTTTA
TGCGAAAAGCTAGCCATGAACTAATAGGATAGCTCAGAGAGATTGCAAGATCTTT

Last Updated on 06.12.2018 16:11