Sequences from confirmation amplicon


Confirmation sequences for line 600D10 and hit At2g26570 are shown.
Line ID 600D10
Locus At2g26570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11301842
Primers used for PCR NF44 - 8474, show primer details
   
Sequence Name 12-K102498-0022-600-D10C-8409
GenBank Accession LN491522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102498-0022-600-D10C-8409
CATTGAAATATCCTGCCCTCCATCTTTCTTTCTCTTTTGTTCATGTTCTGCCCTCCATTT
CCTCAGCTCCTGCTCCACCCCCAACTTCCCTTCTTTGGCCTTCTCAGCCTTTTCAGTTGC
TTCTTTTAATGCCTTCTTTCTCGCATCCATGTCTCTGTTTACTTCTTCCAACTTCTCCAA
GCTTCTCATTTCTGTTTCTTTAGCTTCCTCGATTCTTGAAACCGCTGCTGCTACTCTTGC
GTTCGCGAGTTCTTCTGCCTCGTGAGCACGTTTGCTGAGCTCATAGTACTCTTCTAGCGA
AAGTGTAACGCTTCGTGGAGAATCGGTGTCATTAGCTTTCAATGTTGAA
   
Sequence Name 12-K102499-0022-600-D10C-NF44
GenBank Accession LN491523 [GenBank]
Sequencing primer gene specific primer, NF44: 5'-TCAACATTGAAAGCTAATGACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102499-0022-600-D10C-NF44
ACTATGAGCTCAGCAAACGTGCTCACGAGGCAGAAGAACTCGCGAACGCAAGAGTAGCAG
CAGCGGTTTCAAGAATCGAGGAAGCTAAAGAAACAGAAATGAGAAGCTTGGAGAAGTTGG
AAGAAGTAAACAGAGACATGGATGCGAGAAAGAAGGCATTAAAAGAAGCAACTGAAAAGG
CTGAGAAGGCCAAAGAAGGGAAGTTGGGGGTGGAGCAGGAGCTGAGGAAATGGAGGGCAG
AACATGAACAAAAGAGAAAGAAAGATGGAGGGCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCCAA
ATGGG

Last Updated on 06.12.2018 16:11