Sequences from confirmation amplicon


Confirmation sequences for line 601F12 and hit At1g66700 are shown.
Line ID 601F12
Locus At1g66700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24873581
Primers used for PCR JD10 - 8474, show primer details
   
Sequence Name 49-K027356-022-601-F12C-8409
GenBank Accession LN506712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K027356-022-601-F12C-8409
CATGAAGCCAAATGTGCGGAAATGAAAGCACCATAGAAGGCCTTGAAAGAGATGGCCAAT
GTCTTGGGGTCAAAAGGAAACTCGTTTTTAGCGTGAATGATATCTTCAAAAGCCTCGATT
GTGAACTTCCCATTCTCCTCAATGATTTGCCTTATCTCACCTTGTTCTGCAAAGTAAATT
GAGGTTCTGAAAGTGTCCACCTTCTCTTGCTCGGTGACACCCTTTTTTATATCATAAAAA
AAAAGAAGAAAATGTTAGTTAGTACTTATGCAGTTGTATAATACTATAACGATCAAATTA
TGTACCTGTTTAGCAAGATCGCTAAGAGATTCTCCAATAAAATCCATTACAGTTCCTTTA
GGGGTCTCGGACATCTTAACTCCGTCTCTTAGACACGATCCTAAAAGCAACATCAACCCC
CCGGGCACGAGCTCTTCAGCTCTAGCATCTAAAAGAATTTTGGTGTCGATAGAGTACTGG
TCAAGATACGCTTTCTTGACAGCGGGGTTGAACCCCGTGCACTGCATGTCTTTGTTCCAC
AAGGGAGAGTCGCGGTCCATAATCCCTTTGGGGATTTTGGAGGTGAAATGGAACGCGTAA
CTCATGACTCCGATGTGGAAA
   
Sequence Name 50-K027356-022-601-F12C-JD10
GenBank Accession LN507050 [GenBank]
Sequencing primer gene specific primer, JD10: 5'-TTCTTCCTAAGGAGAGTTTCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K027356-022-601-F12C-JD10
GCGTTCATTTCCCTCCAAATCCCCAAAGGGATTATGGACCGCGACTCTCCCTTGTGGAAC
AAAGACATGCAGTGCACGGGGTTCAACCCCGCTGTCAAGAAAGCGTATCTTGACCAGTAC
TCTATCGACACCAAAATTCTTTTAGATGCTAGAGCTGAAGAGCTCGTGCCCGGGGGGTTG
ATGTTGCTTTTAGGATCGTGTCTAAGAGACGGAGTTAAGATGTCCGAGACCCCTAAAGGA
ACTGTAATGGATTTTATTGGAGAATCTCTTAGCGATCTTGCTAAACAGGTACATAATTTG
ATCGTTATAGTATTATACAACTGCATAAGTACTAACTAACATTTTCTTCTTTTTTTTTAT
GATATAAAAAAGGGTGTCACCGAGCAAGAGAAGGTGGACACTTTCAGAACCTCAATTTAC
TTTGCAGAACAAGGTGAGATAAGGCAAATCATTGAGGAGAATGGGAAGTTCACAATCGAG
GCTTTTGAAGATATCATTCACGCTAAAAACGAGTTTCCTTTTGACCCCAAGACATTGGCC
ATCTCTTTCAAGGCCTTCTATGGTGCTTTCATTTCCGCACATTTTGGCTTCATGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11