Sequences from confirmation amplicon


Confirmation sequences for line 601H08 and hit At5g24790 are shown.
Line ID 601H08
Locus At5g24790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8513044
Primers used for PCR CH64 - 8474, show primer details
   
Sequence Name 27-K105918-0022-601-H08C-CH64
GenBank Accession LN498388 [GenBank]
Sequencing primer gene specific primer, CH64: 5'-CAGGACAATCAGAAGACGAACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105918-0022-601-H08C-CH64
TGATGGCCACGACCTGCGTGCTTCTCTGCGCGGGTCTAGCCGCAGTGTTAAGCAGCACTT
ACAGCATCAAGAAGCCTCTAAACGACGCCGTTTTTGGTGCTCATGGAGATTTCGCCATAT
CCATCAAATACCTAACAATTCTCACAATTTTCATCTTCTCCTTTTTCTTCCACTCCCTCT
CCATCCGTTTCCTAAACCAAGTCGCCATTCTCGTAAACATCCCTAACTTAGACCCTAATC
CTTCTGGTTGCGTCTTCCTCACGTCTGAGCACGTCAGCGAGATGTTCGAAAAGGGTATCT
TTCTTAACACGGTAGGTAACAGGTTGTTCTACGCAGGCTTTTCTCTAATGTTATGGATCT
TTGGTCCCATCCTCGTGTTCTTTAGCGTTTTGGTGATGGTTCTTGTTCTTTCTCACCTCG
ATTTCGTGTCACGCAACAACAATAAAGAAAAACTACGTATAGTGGATATACGTCGTTCCT
CTGATTTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 27-K105919-0022-601-H08C-8409
GenBank Accession LN498389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105919-0022-601-H08C-8409
GCCATTTAAAATCAGAGGAACGACGTATATCCACTATACGTAGTTTTTCTTTATTGTTGT
TGCGTGACACGAAATCGAGGTGAGAAAGAACAAGAACCATCACCAAAACGCTAAAGAACA
CGAGGATGGGACCAAAGATCCATAACATTAGAGAAAAGCCTGCGTAGAACAACCTGTTAC
CTACCGTGTTAAGAAAGATACCCTTTTCGAACATCTCGCTGACGTGCTCAGACGTGAGGA
AGACGCAACCAGAAGGATTAGGGTCTAAGTTAGGGATGTTTACGAGAATGGCGACTTGGT
TTAGGAAACGGATGGAGAGGGAGTGGAAGAAAAAGGAGAAGATGAAAATTGTGAGAATTG
TTAGGTATTTGATGGATATGGCGAAATCTCCATGAGCACCAAAAACGGCGTCGTTTAGAG
GCTTCTTGATGCTGTAAGTGCTGCTTAACACTGCGGCTAGACCCGCGCAGAGAAGCACGC
AGGTCGTGGCCATCAGTGTAGCTCCCATTACTATGTTACGCAGTGTTTGTACCGCCNATA
TGTTCGTCTTCTGATTTGTCCT

Last Updated on 06.12.2018 16:11